Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Efficient Decellularization of Full-Thickness Rat Derived Abdominal Wall to Produce Acellular Biologic Scaffolds for Tissue Reconstruction: Promising Evidence Acquired From in vitro Results

George Skepastianos
† ,
Panagiotis Mallis
*,† ORCID logo ,
Epameinondas Kostopoulos
ORCID logo ,
Efstathios Michalopoulos
ORCID logo ,
Vasileios Skepastianos
,
Chrysoula Palazi
,
Lucia Pannuto
,
Gerasimos Tsourouflis
†
Equally contributed to this work as first authors
Version 1 : Received: 6 July 2023 / Approved: 7 July 2023 / Online: 7 July 2023 (10:24:10 CEST)

A peer-reviewed article of this Preprint also exists.

Skepastianos, G.; Mallis, P.; Kostopoulos, E.; Michalopoulos, E.; Skepastianos, V.; Palazi, C.; Pannuto, L.; Tsourouflis, G. Efficient Decellularization of the Full-Thickness Rat-Derived Abdominal Wall to Produce Acellular Biologic Scaffolds for Tissue Reconstruction: Promising Evidence Acquired from In Vitro Results. Bioengineering 2023, 10, 913. Skepastianos, G.; Mallis, P.; Kostopoulos, E.; Michalopoulos, E.; Skepastianos, V.; Palazi, C.; Pannuto, L.; Tsourouflis, G. Efficient Decellularization of the Full-Thickness Rat-Derived Abdominal Wall to Produce Acellular Biologic Scaffolds for Tissue Reconstruction: Promising Evidence Acquired from In Vitro Results. Bioengineering 2023, 10, 913.

Abstract

Background: Functional restoration of abdominal wall defects represent one of the fundamental challenges of reconstructive surgery. Synthetic grafts or crosslinked animal-derived biological grafts are characterized by significant adverse reactions, which are mostly observed after their implantation. The aim of this study was to evaluate the efficacy of the decellularization protocol to produce a completely acellular full-thickness abdominal wall scaffold. Methods: Full-thickness abdominal wall samples were harvested from Wistar rats and submitted to a 3 cylce decellularization process. Histological, biochemical and DNA quantification analyses were applied to evaluate the effect of the decellularization protocol. Mechanical testing and immunogenicity assessment were also performed. Results: Histological, biochemical and DNA analysis results showed the efficient decellularization abdominal wall samples after the 3rd cycle. Decellularized abdominal wall scaffolds were characterized by good biochemical and mechanical properties Conclusion: The data presented herein confirm the effective production of rat-derived full-thickness abdominal wall scaffold. Expanding this approach will allow the exploitation of the capacity of the proposed decellularization protocol in producing acellular abdominal wall scaffolds from larger animal models or human cadaveric donors. In this way, the utility of biological scaffolds with preserved in vivo remodelling properties may be one step closer to its application in clinical studies.

Keywords

Abdominal wall; decellularization; hernia; tissue reconstruction; biomechanical analysis; full-thickness abdominal wall scaffold

Subject

Engineering, Bioengineering

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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