Zhang, Y.; Xu, Y.; Jiang, S.; Sun, S.; Zhang, J.; Luo, J.; Cao, Q.; Zheng, W.; Meurens, F.; Chen, N.; Zhu, J. Multiple Porcine Innate Immune Signaling Pathways Are Involved in the Anti-PEDV Response. Viruses2023, 15, 1629.
Zhang, Y.; Xu, Y.; Jiang, S.; Sun, S.; Zhang, J.; Luo, J.; Cao, Q.; Zheng, W.; Meurens, F.; Chen, N.; Zhu, J. Multiple Porcine Innate Immune Signaling Pathways Are Involved in the Anti-PEDV Response. Viruses 2023, 15, 1629.
Zhang, Y.; Xu, Y.; Jiang, S.; Sun, S.; Zhang, J.; Luo, J.; Cao, Q.; Zheng, W.; Meurens, F.; Chen, N.; Zhu, J. Multiple Porcine Innate Immune Signaling Pathways Are Involved in the Anti-PEDV Response. Viruses2023, 15, 1629.
Zhang, Y.; Xu, Y.; Jiang, S.; Sun, S.; Zhang, J.; Luo, J.; Cao, Q.; Zheng, W.; Meurens, F.; Chen, N.; Zhu, J. Multiple Porcine Innate Immune Signaling Pathways Are Involved in the Anti-PEDV Response. Viruses 2023, 15, 1629.
Abstract
Porcine epidemic diarrhea virus (PEDV) has caused great damage to the global pig industry. The innate immunity plays a significant role in resisting viral infection, however, the exact role of innate immunity in the anti-PEDV response has not been fully elucidated. In this study, we observed that various porcine innate immune signaling adaptors are involved in the anti-PEDV activity. Among these, TRIF and MAVS showed the strongest anti-PEDV activity. The endogenous TRIF, MAVS and STING were selected for further examination of anti-PEDV activity. Agonist stimulation experiments showed that TRIF, MAVS and STING signaling all have obvious anti-PEDV activity. The siRNA knockdown assay showed that TRIF, MAVS and STING are also all involved in anti-PEDV response, and their remarkable effects on PEDV replication were confirmed in TRIF-/-, MAVS-/- and STING-/- Vero cells. For further verification, the anti-PEDV activity of TRIF, MAVS and STING could be reproduced in porcine IPEC-DQ cells. In summary, this study reveals that multiple pattern recognition receptor (PRR) signaling pathways of porcine innate immunity play an important role in the anti-PEDV infection, providing new and useful antiviral knowledge for prevention and control of PEDV spreading.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
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