These two authors contributed equally as co-corresponding authors.
Version 1
: Received: 4 July 2023 / Approved: 4 July 2023 / Online: 5 July 2023 (02:29:07 CEST)
How to cite:
Kim, H.E.; Kim, E.Y.; Ahn, J.; Mo, S. Cell Death Inhibition Effect of Antioxidant Activity by 630 and 850 nm LEDs in RAW264.7 cells. Preprints2023, 2023070212. https://doi.org/10.20944/preprints202307.0212.v1
Kim, H.E.; Kim, E.Y.; Ahn, J.; Mo, S. Cell Death Inhibition Effect of Antioxidant Activity by 630 and 850 nm LEDs in RAW264.7 cells. Preprints 2023, 2023070212. https://doi.org/10.20944/preprints202307.0212.v1
Kim, H.E.; Kim, E.Y.; Ahn, J.; Mo, S. Cell Death Inhibition Effect of Antioxidant Activity by 630 and 850 nm LEDs in RAW264.7 cells. Preprints2023, 2023070212. https://doi.org/10.20944/preprints202307.0212.v1
APA Style
Kim, H.E., Kim, E.Y., Ahn, J., & Mo, S. (2023). Cell Death Inhibition Effect of Antioxidant Activity by 630 and 850 nm LEDs in RAW264.7 cells. Preprints. https://doi.org/10.20944/preprints202307.0212.v1
Chicago/Turabian Style
Kim, H.E., Jin-Chul Ahn and SangJoon Mo. 2023 "Cell Death Inhibition Effect of Antioxidant Activity by 630 and 850 nm LEDs in RAW264.7 cells" Preprints. https://doi.org/10.20944/preprints202307.0212.v1
Abstract
Phototherapy is an efficient strategy to respond to oxidative stress and has been in the spotlight in various therapeutic fields through its biological effect, but the exact molecular mechanism has not yet been established. As a result of irradiating RAW264.7 cells with a 630 nm or 850 nm LED with an energy intensity of 0.01J/cm2, cell viability was increased depend on the irradiation time. After H2O2 was added, 630 nm or 850 nm LEDs were irradiated for 10 min or 40 min to increase cell viability by about 1.7- or 1.6-fold, respectively. In addition, 630nm or 850nm LED irradiation showed a significant ROS scavenging effect and significantly reduced TUNEL-positive cells by 45.7% and 37.8%, respectively, compared to cells treated only with H2O2. In the result of flow cytometry, the number of cells in the late apoptosis stage was about 2.3- and 1.8-fold lower than that of the cells treated with H2O2 only, but there was no significance. The Bax/Bcl-2 ratio of cells irradiated with 630 nm or 850 nm LED was significantly lower than that of cells treated with H2O2 only, and the expression of procaspase-3 and cleaved PARP was also significantly expressed in the direction of suppressing cell death. In conclusion, the ROS scavenging activity by 630 nm or 850 nm LED irradiation, leading the expression of cell death pathway proteins in the direction of inhibiting cell death.
Keywords
oxidative stress; photobiomodulation; LED irradiation; cell death
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
