Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Modified Lactoperoxidase System as a Promising Anticaries Agent: In Vitro Studies on Streptococcus mutans Biofilms

Marcin Magacz
ORCID logo ,
Sergio Alatorre-Santamaría
,
Karolina Kędziora
,
Kacper Klasa
ORCID logo ,
Paweł Mamica
,
Wiktoria Pepasińska
,
Magdalena Lebiecka
,
Wirginia Krzyściak
* ,
Dorota Kościelniak
,
Elżbieta Pamuła
Version 1 : Received: 30 June 2023 / Approved: 3 July 2023 / Online: 3 July 2023 (12:58:53 CEST)

A peer-reviewed article of this Preprint also exists.

Magacz, M.; Alatorre-Santamaría, S.; Kędziora, K.; Klasa, K.; Mamica, P.; Pepasińska, W.; Lebiecka, M.; Kościelniak, D.; Pamuła, E.; Krzyściak, W. Modified Lactoperoxidase System as a Promising Anticaries Agent: In Vitro Studies on Streptococcus mutans Biofilms. Int. J. Mol. Sci. 2023, 24, 12136. Magacz, M.; Alatorre-Santamaría, S.; Kędziora, K.; Klasa, K.; Mamica, P.; Pepasińska, W.; Lebiecka, M.; Kościelniak, D.; Pamuła, E.; Krzyściak, W. Modified Lactoperoxidase System as a Promising Anticaries Agent: In Vitro Studies on Streptococcus mutans Biofilms. Int. J. Mol. Sci. 2023, 24, 12136.

Abstract

The lactoperoxidase (LPO) system shows promise in the prevention of dental caries, a common chronic disease. This system has antimicrobial properties and is part of the non-specific antimicrobial immune system. Understanding the efficacy of the LPO system in the fight against biofilms could provide information on alternative strategies for the prevention and treatment of caries. In this study, the enzymatic system was modified using four different (pseudo)halide substrates (thiocyanate, thiocyanate-iodide mixture, selenocyanate, and iodide). The study evaluated the effects of such modifications on the ability of Streptococcus mutans to form a biofilm, the synthesis of insoluble polysaccharides, the synthesis of lactate, the consumption of glucose and sucrose, the concentrations of intracellular NAD+ and NADH and the efficiency of transmembrane glucose transport by phosphoenolpuryvate PEP-carbohydrate phosphotransferase (PTS). The results showed that the LPO-iodide system had the strongest inhibitory effect on biofilm growth and lactate synthesis (complete inhibition). This was associated with an increase in the NAD+/NADH ratio and an inhibition of glucose PTS activity. The LPO-selenocyanate system showed a moderate inhibitory effect on biofilm biomass growth and lactate synthesis. The other systems showed relatively small inhibition of lactate synthesis and glucose PTS but no effect on the growth of biofilm biomass. This study provides a basis for further research on the use of alternative substrates of the LPO system, particularly the LPO-iodide system, in the prevention and control of biofilm-related diseases.

Keywords

lactoperoxidase; Streptococcus mutans; biofilm; dental caries; lactoperoxidase system modulators; cariogenic biofilm

Subject

Medicine and Pharmacology, Pharmacy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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