Version 1
: Received: 25 June 2023 / Approved: 26 June 2023 / Online: 26 June 2023 (09:12:29 CEST)
How to cite:
Oliel, M.; Mastai, Y. Isothermal Titration Calorimetry: The Heat of Dilution, Racemization, and What Lies In Between. Preprints2023, 2023061763. https://doi.org/10.20944/preprints202306.1763.v1
Oliel, M.; Mastai, Y. Isothermal Titration Calorimetry: The Heat of Dilution, Racemization, and What Lies In Between. Preprints 2023, 2023061763. https://doi.org/10.20944/preprints202306.1763.v1
Oliel, M.; Mastai, Y. Isothermal Titration Calorimetry: The Heat of Dilution, Racemization, and What Lies In Between. Preprints2023, 2023061763. https://doi.org/10.20944/preprints202306.1763.v1
APA Style
Oliel, M., & Mastai, Y. (2023). Isothermal Titration Calorimetry: The Heat of Dilution, Racemization, and What Lies In Between. Preprints. https://doi.org/10.20944/preprints202306.1763.v1
Chicago/Turabian Style
Oliel, M. and Yitzhak Mastai. 2023 "Isothermal Titration Calorimetry: The Heat of Dilution, Racemization, and What Lies In Between" Preprints. https://doi.org/10.20944/preprints202306.1763.v1
Abstract
Chiral interactions play a crucial role in both chemistry and biology. Understanding the behavior of chiral molecules and their interactions with other molecules is essential, and chiral interactions in solutions are particularly important for studying chiral compounds. Chirality influences the physical and chemical properties of molecules, including solubility, reactivity, and biological activity. In this work, we used Isothermal Titration Calorimetry (ITC), a powerful technique for studying molecular interactions, including chiral interactions in solutions. We conducted a series of ITC measurements to investigate the heat of dilution and the heat of racemization of several amino acids (Asn, His, Ser, Ala, Met, and Phe). We also performed ITC measurements under different solute concentrations and temperatures to examine the effects of these parameters on chiral interactions, as well as the heat of dilution and racemization. The results of our measurements indicated that the heat of dilution, specifically the interactions between the solvent (water) and solute (chiral molecules), had a significant impact compared to the chiral interactions in the solution, which were found to be negligible. This suggests that the interactions between chiral molecules and the solvent play a more dominant role in determining the overall behavior and properties of the system. By studying chiral interactions in solutions, we can gain valuable insights into the behavior of chiral compounds, which can have implications in various fields, including drug design, chemical synthesis, and biological processes.
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
