Version 1
: Received: 6 June 2023 / Approved: 25 June 2023 / Online: 25 June 2023 (04:52:06 CEST)
How to cite:
Changjalern, M.; Hongtrakul, V.; Ohmido, N.; Srinophakun, P. Characterization and Classification of Ty1-copia Retrotransposons in Jatropha curcas L.. Preprints2023, 2023061711. https://doi.org/10.20944/preprints202306.1711.v1
Changjalern, M.; Hongtrakul, V.; Ohmido, N.; Srinophakun, P. Characterization and Classification of Ty1-copia Retrotransposons in Jatropha curcas L.. Preprints 2023, 2023061711. https://doi.org/10.20944/preprints202306.1711.v1
Changjalern, M.; Hongtrakul, V.; Ohmido, N.; Srinophakun, P. Characterization and Classification of Ty1-copia Retrotransposons in Jatropha curcas L.. Preprints2023, 2023061711. https://doi.org/10.20944/preprints202306.1711.v1
APA Style
Changjalern, M., Hongtrakul, V., Ohmido, N., & Srinophakun, P. (2023). Characterization and Classification of Ty1-<em>copia</em> Retrotransposons in <em>Jatropha curcas</em> L.. Preprints. https://doi.org/10.20944/preprints202306.1711.v1
Chicago/Turabian Style
Changjalern, M., Nobuko Ohmido and Penjit Srinophakun. 2023 "Characterization and Classification of Ty1-<em>copia</em> Retrotransposons in <em>Jatropha curcas</em> L." Preprints. https://doi.org/10.20944/preprints202306.1711.v1
Abstract
Jatropha curcas is a potential energy crop and has been identified as a significant resource for biodiesel. Retrotransposons occupy the plant genome in a large proportion, so the specific retrotransposon characterization will help improve understanding of the Jatropha genome evolution and organization. This research aimed to characterize and classify Ty1-copia retrotransposons in J. curcas using specific gene sequence and to detect the distribution of Ty1-copia in J. curcas and J. integerrima chromosomes using florescence in situ hybridization (FISH) method. Ty1-copia sequences from J. curcas were isolated using three degenerate primer sets specific to the conserved Ty1-copia reverse transcriptase gene for polymerase chain reaction (PCR). FISH technique using the Ty1-copia probes was used to map physically on J. curcas and J. integerrima chromosomes. Altogether, 164 Ty1-copia sequences were obtained to use for the phylogenetic analysis and were classified into the four families of TAR, Angela, Ale and Bianca. Physical map using FISH method of Angela and Ale families on J. curcas and J. integerrima chromosomes exhibited biases for specific regions around the centromeres or at the chromosome terminal regions. The study indicates the high variation of the Ty1-copia elements in the genome of Jatropha. These results will enhance understanding of retrotransposon chromosomal distribution and evolution of the J. curcas genome and also promotion of retrotransposon possible utilization in J. curcas improvement projects using retrotransposon-based markers.
Keywords
florescence in situ hybridization; Jatropha curcas; phylogenetic tree analysis; Ty1-copia retrotransposons
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
