Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds With Different Additives of ATBC and OLA Plasticizers

Marianna Trebuňová
* ORCID logo ,
Patrícia Petroušková
ORCID logo ,
Alena Findrik Balogová
ORCID logo ,
Gabriela Ižaríková
,
Peter Horňak
ORCID logo ,
Darina Bačenková
ORCID logo ,
Jana Demeterová
ORCID logo ,
Jozef Živčák
Version 1 : Received: 22 June 2023 / Approved: 23 June 2023 / Online: 23 June 2023 (12:05:49 CEST)

A peer-reviewed article of this Preprint also exists.

Trebuňová, M.; Petroušková, P.; Balogová, A.F.; Ižaríková, G.; Horňak, P.; Bačenková, D.; Demeterová, J.; Živčák, J. Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers. J. Funct. Biomater. 2023, 14, 412. Trebuňová, M.; Petroušková, P.; Balogová, A.F.; Ižaríková, G.; Horňak, P.; Bačenková, D.; Demeterová, J.; Živčák, J. Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers. J. Funct. Biomater. 2023, 14, 412.

Abstract

The aim of this study was to analyse the biocompatibility of a material based on lactic acid (PLA)/polyhydroxybutyrate(PHB)/thermoplastic starch (TPS) in combination with additives (plasticizers: acetyltributyl citrate (ATBC) and oligomeric lactic acid ( OLA)). Three types of materials were tested: I: PLA/PHB/TPS + 25% OLA, II: PLA/PHB/TPS + 30% ATBC, III: PLA/PHB/TPS + 30% OLA. The 3D printing itself was preceded by the creation of filaments from granulate and the design of samples. A filament production machine – Composer 450 (3devo; The Netherlands) was used to produce the filaments. Experimental samples were produced by FDM technology on a 3D printer DeltiQ2 (Trilab, Czech Republic). The evaluation of the biocompatibility of the materials included cytotoxicity tests such as the monitoring of viability, proliferation, morphology of cells, and their settlement on the surface of the materials. The cell line 7F2 osteoblasts (Mus musculus) was used in the experiments. Based on the tests, results confirmed the significant influence of plasticizers and their proportions in the mixtures. PLA/PHB/ATBC + 25% OLA was evaluated as the optimal material for biocompatibility with 7F2 osteoblasts. The tested biomaterials have the potential for further investigation, with a possible change in the proportion of the plasticizer, which has a fundamental impact on their biological properties.

Keywords

biocompatibility; PLA/PHB/TPS polymer scaffolds; plasticizers

Subject

Engineering, Bioengineering

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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