Version 1
: Received: 22 June 2023 / Approved: 23 June 2023 / Online: 23 June 2023 (10:50:59 CEST)
How to cite:
Ng, W. Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry. Preprints2023, 2023061658. https://doi.org/10.20944/preprints202306.1658.v1
Ng, W. Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry. Preprints 2023, 2023061658. https://doi.org/10.20944/preprints202306.1658.v1
Ng, W. Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry. Preprints2023, 2023061658. https://doi.org/10.20944/preprints202306.1658.v1
APA Style
Ng, W. (2023). Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry. Preprints. https://doi.org/10.20944/preprints202306.1658.v1
Chicago/Turabian Style
Ng, W. 2023 "Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry" Preprints. https://doi.org/10.20944/preprints202306.1658.v1
Abstract
Quantitative polymerase chain reaction (qPCR) is the gold standard approach for detecting a variety of pathogens either via probe-based or SYBR green detection chemistry. Generally, probe-based detection is more specific than SYBR green chemistry. This report describes work done in using a FAM probe targeting a specific segment of the human KRAS gene for detecting presence and relative abundance of the gene in human gastric CloTest samples. Results indicate ineffective detection of the human KRAS gene in samples that are shown to harbor the gene through a similar qPCR assay using SYBR green detection chemistry. Such observations point to inability of the designed FAM probe to bind to target segment of the human KRAS gene that suggests a region of rapid genomic evolution that require close surveillance in the public health community. Given its rapid genomic evolution, this probe region may be an important region in the human KRAS gene playing critical functional roles in either molecular recognition or other structural functions. Overall, human KRAS gene may be undergoing rapid genomic evolution, and qPCR probes targeting specific segment of the gene may be rendered ineffective in short time clinically, thereby, making SYBR green qPCR assay a more reliable choice.
Keywords
qPCR assay; probe-based detection; SYBR green; human KRAS gene; human gastric CloTest samples
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
