Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Preparation of High Selenized Gastrodia elata Blume Polysaccharides and Its Immunomodulatory Effects on RAW 264.7 Macrophages and Cyclophosphamide-treated Mice

Version 1 : Received: 21 June 2023 / Approved: 21 June 2023 / Online: 21 June 2023 (13:32:14 CEST)

A peer-reviewed article of this Preprint also exists.

Ma, F., Wen, Q., Deng, Q., Lu, Y., Zhang, B., Cheng, Y., & Xu, S. (2023). Preparation of High Selenized Gastrodia elata Blume Polysaccharides and Its Immunomodulatory Effects on RAW 264.7 Macrophages and Cyclophosphamide-treated Mice. Pakistan Journal of Zoology. Ma, F., Wen, Q., Deng, Q., Lu, Y., Zhang, B., Cheng, Y., & Xu, S. (2023). Preparation of High Selenized Gastrodia elata Blume Polysaccharides and Its Immunomodulatory Effects on RAW 264.7 Macrophages and Cyclophosphamide-treated Mice. Pakistan Journal of Zoology.

Abstract

Selenium (Se) is an essential trace element for human beings and Se supplementation is of great significance. In this paper, high selenized Gastrodia elata Blume Polysaccharides (SeGEP) were obtained through pre-sulfation before conducting the selenylation modification process, which owned a maximum Se content reach to 2.29%. UV, FT-IR, NMR, SEM, EDX, HPLC, size-exclusion chromatography and periodate oxidation analysis showed that the seleninic group had been successfully introduced into GEP with the substitution position at C-6 of glucose. Immunostimulating assays were conducted in vitro and in vivo on RAW 264.7 cells and cyclophosphamide (CTX)-induced mice model, respectively. The results showed that Se-GEP enhanced RAW264.7 cells proliferation, cell phagocytosis, and secretion of nitric oxide (NO) and interleukin (IL)-1β factors. In addition, Se-GEP enhanced the immunologic activity of CTX-treated mice by increasing the spleen and thymus indices, enhancing the phagocytic function, and activating both Th1 and Th2 lymphocytes. The current outcome also suggested that Se-GEP with covalently bonding Se group possessing increased safety and has a potential dietary selenium nutrient for improving human immunity.

Keywords

Gastrodia elata Blume; High selenized polysaccharide; Modification; Immunomodulatory activity

Subject

Chemistry and Materials Science, Food Chemistry

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