Version 1
: Received: 21 June 2023 / Approved: 21 June 2023 / Online: 21 June 2023 (05:31:05 CEST)
How to cite:
Kim, K.; Joo, G.; Jeong, K.; Lee, Y.; Hong, D.; Jo, H. Molecular Diet Analysis of Asian Clam for Estuarine Biodiversity Monitoring: A Case Study of Nakdong River Estuary. Preprints2023, 2023061484. https://doi.org/10.20944/preprints202306.1484.v1
Kim, K.; Joo, G.; Jeong, K.; Lee, Y.; Hong, D.; Jo, H. Molecular Diet Analysis of Asian Clam for Estuarine Biodiversity Monitoring: A Case Study of Nakdong River Estuary. Preprints 2023, 2023061484. https://doi.org/10.20944/preprints202306.1484.v1
Kim, K.; Joo, G.; Jeong, K.; Lee, Y.; Hong, D.; Jo, H. Molecular Diet Analysis of Asian Clam for Estuarine Biodiversity Monitoring: A Case Study of Nakdong River Estuary. Preprints2023, 2023061484. https://doi.org/10.20944/preprints202306.1484.v1
APA Style
Kim, K., Joo, G., Jeong, K., Lee, Y., Hong, D., & Jo, H. (2023). Molecular Diet Analysis of Asian Clam for Estuarine Biodiversity Monitoring: A Case Study of Nakdong River Estuary. Preprints. https://doi.org/10.20944/preprints202306.1484.v1
Chicago/Turabian Style
Kim, K., Donghyun Hong and Hyunbin Jo. 2023 "Molecular Diet Analysis of Asian Clam for Estuarine Biodiversity Monitoring: A Case Study of Nakdong River Estuary" Preprints. https://doi.org/10.20944/preprints202306.1484.v1
Abstract
eDNA extracted from the gut contents of filter feeders with unique feeding habits can be used to identify biodiversity in aquatic ecosystems. In this study, we used eDNA from the gut contents of the clam Corbicula fluminea to examine estuarine ecosystem biodiversity. The field survey was conducted at three study sites in the Nakdong River Estuary, which is characterised by closed estuarine features resulting from the presence of an estuarine barrage. The collected C. fluminea samples were dissected to separate the gut contents, and the extracted eDNA was amplified using the 18S V9 primer targeting all eukaryotes. The amplified DNA was sequenced using next generation sequencing (NGS) techniques, and BLASTn was performed based on the NCBI database. We obtained 21 unique operational taxonomic units (OTUs), including fish (approximately 9.52%), copepods (approximately 14.29%), and green algae (approximately 23.81%), which represented a wide range of habitats such as marine, brackish, freshwater, and terrestrial environments. These results suggest that various organisms living in aquatic ecosystems can be identified through eDNA from the gut contents of C. fluminea and support the potential application of bivalves as eDNA samplers in diverse aquatic ecosystems.
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
