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Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations
Liu, Y.; Lin, L.; Liu, Y.; Mo, Q.; Zhang, D.; Li, W.; Xiong, X.; Yu, X.; Li, Y. Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations. Preprints2023, 2023061460. https://doi.org/10.20944/preprints202306.1460.v1
APA Style
Liu, Y., Lin, L., Liu, Y., Mo, Q., Zhang, D., Li, W., Xiong, X., Yu, X., & Li, Y. (2023). Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations. Preprints. https://doi.org/10.20944/preprints202306.1460.v1
Chicago/Turabian Style
Liu, Y., Xiaoying Yu and Yanlin Li. 2023 "Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations" Preprints. https://doi.org/10.20944/preprints202306.1460.v1
Abstract
The basic helix-loop-helix (bHLH) transcription factor family is the second-largest transcription factor in plants. Members of this family are involved in the process of growth and development, secondary metabolic biosynthesis, signal transduction, and plant resistance. Loropetalum chinense var. rubrum is a critical woody plant with higher ornamental and economic values, which used as ornamental architecture and traditional Chinese herbal medicine plant. However, the bHLH transcription factor in the L. chinense var. rubrum (Loropetalum chinense var. Rubrum) have yet to be systematically demonstrated, and their role in anthocyanin biosynthesis remains secret. Here, we identified 165 potential LcbHLHs genes by two methods, and they were unequally distributed on chromosomes 1 to 12 of the L. chinense genome. Based on a phylogenetic comparison with proteins from Arabidopsis, these bHLH proteins were categorized into 21 subfamilies. Most LcbHLHs in a given subfamily had similar gene structures and conserved motifs. The gene ontology annotation and cis-elements predicted that the LcbHLHs had many molecular functions and were involved in plant growth processes, including flavonoid and anthocyanin biosynthetic processes, etc. Transcriptomic analysis revealed different expression patterns among different tissues and cultivars in L. chinense. Many LcbHLHs were expressed in leaves, and only a few genes were highly expressed in flowers. Six LcbHLH genes differentially expressed between species and periods in color variation, which may play a key role in anthocyanin synthesis. These further functional predictions of LcbHLHs were established by quantitative real-time PCR analysis and protein-protein interaction network. This study identified the six potential bHLH genes related to anthocyanin in L. chinense and created a solid framework for future research on the function and evolution of bHLHs.
Keywords
L. chinense var. rubrum.; bHLH transcription factor; expression analyses; anthocyanin
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
