Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Anti-Inflammatory Effect and Signaling Mechanism of Glycine max Hydrolyzed With Enzymes From Bacillus velezensis KMU01 in a Dextran Sulfate Sodium-Induced Colitis Mouse Model

Seung-Hyeon Lee
,
Ha-Rim Kim
,
Eun-Mi Noh
,
Jae Young Park
,
Mi-Sum Kwak
,
Ye-Jin Jung
,
Hee-Jong Yang
ORCID logo ,
Myeong-Seon Ryu
,
Hyangyim Seo
,
Hansu Jang
,
Seon-Young Kim
* ORCID logo ,
Mi Hee Park
*
Version 1 : Received: 19 June 2023 / Approved: 19 June 2023 / Online: 19 June 2023 (07:13:51 CEST)

A peer-reviewed article of this Preprint also exists.

Lee, S.-H.; Kim, H.-R.; Noh, E.-M.; Park, J.Y.; Kwak, M.-S.; Jung, Y.-J.; Yang, H.-J.; Ryu, M.S.; Seo, H.-Y.; Jang, H.; Kim, S.-Y.; Park, M.H. Anti-Inflammatory Effect and Signaling Mechanism of Glycine max Hydrolyzed with Enzymes from Bacillus velezensis KMU01 in a Dextran-Sulfate-Sodium-Induced Colitis Mouse Model. Nutrients 2023, 15, 3029. Lee, S.-H.; Kim, H.-R.; Noh, E.-M.; Park, J.Y.; Kwak, M.-S.; Jung, Y.-J.; Yang, H.-J.; Ryu, M.S.; Seo, H.-Y.; Jang, H.; Kim, S.-Y.; Park, M.H. Anti-Inflammatory Effect and Signaling Mechanism of Glycine max Hydrolyzed with Enzymes from Bacillus velezensis KMU01 in a Dextran-Sulfate-Sodium-Induced Colitis Mouse Model. Nutrients 2023, 15, 3029.

Abstract

The purpose of this study was to investigate the effect that Glycine max hydrolyzed with enzymes from Bacillus velezensis KMU01 has on dextran sulfate sodium (DSS)-induced colitis in mice. Hydrolysis improves functional health through the bioconversion of raw materials and increase in intestinal absorption rate and antioxidants. Therefore, G. max was hydrolyzed in this study using a food-derived microorganism, and its anti-inflammatory effect observed. Enzymatically hydrolyzed G. max (EHG) was orally administered once daily for four weeks before DSS treatment. Colitis was induced in mice through consumption of 5% (w/v) DSS in drinking water for eight days. Results showed that EHG treatment significantly alleviated DSS-induced body weight loss and decreased the disease activity index and colon length. In addition, EHG markedly reduced tumor necrosis factor-a, interleukin (IL)-1b, and IL-6 production, and increased that of IL-10. EHG improved DSS-induced histological changes and intestinal epithelial barrier integrity in mice. Moreover, we found that the abundance of 15 microorganisms changed significantly; that of Proteobacteria and Escherichia coli, which are upregulated in patients with Crohn’s disease and ulcerative colitis, decreased after EHG treatment. These results suggest that EHG has a protective effect against DSS-induced colitis and is a potential candidate for colitis treatment.

Keywords

Enzymatic hydrolysis; Glycine max; Bacillus velezensis KMU01; inflammation; dextran sulfate sodium; colitis

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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