Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Magnetic Molecularly Imprinted Chitosan Combined with a Paper-Based Analytical Device for the Smartphone Discrimination of Tryptophan Enantiomers

Abdelhafid Karrat
ORCID logo ,
Juan José García-Guzmán
,
José María Palacios Santander
* ORCID logo ,
Aziz Amine
* ,
Laura Cubillana-Aguilera
ORCID logo
Version 1 : Received: 16 June 2023 / Approved: 16 June 2023 / Online: 16 June 2023 (14:00:25 CEST)

A peer-reviewed article of this Preprint also exists.

Karrat, A.; García-Guzmán, J.J.; Palacios-Santander, J.M.; Amine, A.; Cubillana-Aguilera, L. Magnetic Molecularly Imprinted Chitosan Combined with a Paper-Based Analytical Device for the Smartphone Discrimination of Tryptophan Enantiomers. Biosensors 2023, 13, 830. Karrat, A.; García-Guzmán, J.J.; Palacios-Santander, J.M.; Amine, A.; Cubillana-Aguilera, L. Magnetic Molecularly Imprinted Chitosan Combined with a Paper-Based Analytical Device for the Smartphone Discrimination of Tryptophan Enantiomers. Biosensors 2023, 13, 830.

Abstract

The chiral recognition of enantiomers is a topic of great importance. In this study, a straightforward and effective chiral detection strategy was developed for distinguishing between tryptophan (TRP) enantiomers. The approach involved the preparation of a magnetic molecularly imprinted chitosan (MMIC) for the sample preparation, which was combined with a nitrocellulose membrane (paper-based analytical device, PAD) integrated with D-TRP covalently-grafted polymethacrylic acid (PAD_PMA_D-TRP). The discrimination between TRP enantiomers was achieved using AuNPs as a colorimetric probe. Indeed, the presence of D-TRP rapidly induced the aggregation of AuNPs due to its strong affinity to PAD-PMA_D-TRP, resulting in a noticeable color change of the AuNPs from red to purple. On the other hand, L-TRP did not induce any color change. The chiral analysis could be easily performed with the naked eye and/or a smartphone. The developed method exhibited a detection limit of 3.3 µM and it was successfully applied to detect D-TRP in serum samples, demonstrating good recovery rates. The proposed procedure is characterized by its simplicity, cost-effectiveness, rapidity, and ease of operation.

Keywords

Chitosan; magnetic molecularly imprinted polymer; enantiomers discrimination; tryp-tophan; smartphone; paper-based analytical device

Subject

Chemistry and Materials Science, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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