Version 1
: Received: 14 June 2023 / Approved: 14 June 2023 / Online: 14 June 2023 (09:02:51 CEST)
How to cite:
Zhu, B.; Liu, H. A Method to Fast Ab Initio Cryo-Electron Microscopy Initial Volume Reconstruction. Preprints2023, 2023061023. https://doi.org/10.20944/preprints202306.1023.v1
Zhu, B.; Liu, H. A Method to Fast Ab Initio Cryo-Electron Microscopy Initial Volume Reconstruction. Preprints 2023, 2023061023. https://doi.org/10.20944/preprints202306.1023.v1
Zhu, B.; Liu, H. A Method to Fast Ab Initio Cryo-Electron Microscopy Initial Volume Reconstruction. Preprints2023, 2023061023. https://doi.org/10.20944/preprints202306.1023.v1
APA Style
Zhu, B., & Liu, H. (2023). A Method to Fast Ab Initio Cryo-Electron Microscopy Initial Volume Reconstruction. Preprints. https://doi.org/10.20944/preprints202306.1023.v1
Chicago/Turabian Style
Zhu, B. and Hongrong Liu. 2023 "A Method to Fast Ab Initio Cryo-Electron Microscopy Initial Volume Reconstruction" Preprints. https://doi.org/10.20944/preprints202306.1023.v1
Abstract
Single particle analysis with cryogenic electron microscopy (cryo-EM) can determine high resolution structures of macromolecular assemblies. To reconstruct final three-dimension(3D) density map, a reliable initial model is essential and ab-initio reconstruction usually can avoid model bias. The grand challenge for ab-initio reconstruction is to alignment two-dimension(2D) low signal-to-noise ratio(SNR) of particle images with random and unknown parameters. Here we formulate a fast ab initio reconstruction method(named AlignFM) based on Fourier Mellin transform and particle-swarm intelligence optimization(PSO) algorithm. It enables simultaneous 2D alignment and 3D refinement, which can fast generate a reliable 3D map. After experiments on several real datasets. AlignFM shows high performance on fast ab initio reconstruction with a small amount of data.
Keywords
Ab initio reconstruction; Simultaneous 2D alignment and 3D refinement; AlignFM; Fourier Mellin transform; Particle swarm intelligence optimization
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
