Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Spectral Characterization, Microscopic Images, and Antimicrobial Activity of Chenodeoxycholic Acid Complexes with Zn(II), Mg(II), and Ca(II) Ions

Abdulrahman A. Almehizia
ORCID logo ,
Mohamed A. Al-Omar
ORCID logo ,
Ahmed M. Naglah
* ,
Mashooq A. Bhat
ORCID logo ,
Fhdah S. Alanazi
,
Fatimah A. Alotaibi
,
Moamen S. Refat
,
Abdel Majid A. Adam
Version 1 : Received: 8 June 2023 / Approved: 8 June 2023 / Online: 8 June 2023 (11:28:03 CEST)

How to cite: Almehizia, A.A.; Al-Omar, M.A.; Naglah, A.M.; Bhat, M.A.; Alanazi, F.S.; Alotaibi, F.A.; Refat, M.S.; Adam, A.M.A. Spectral Characterization, Microscopic Images, and Antimicrobial Activity of Chenodeoxycholic Acid Complexes with Zn(II), Mg(II), and Ca(II) Ions. Preprints 2023, 2023060643. https://doi.org/10.20944/preprints202306.0643.v1 Almehizia, A.A.; Al-Omar, M.A.; Naglah, A.M.; Bhat, M.A.; Alanazi, F.S.; Alotaibi, F.A.; Refat, M.S.; Adam, A.M.A. Spectral Characterization, Microscopic Images, and Antimicrobial Activity of Chenodeoxycholic Acid Complexes with Zn(II), Mg(II), and Ca(II) Ions. Preprints 2023, 2023060643. https://doi.org/10.20944/preprints202306.0643.v1

Abstract

Chenodeoxycholic acid (CA) is a naturally occurring bile acid that is produced in the liver from cholesterol. Three CA complexes using Zn(II), Mg(II), and Ca(II) ions were synthesized to examine the chelation tendencies of CA towards these metal ions. The complexation reaction of CA with the metal ions under investigation was conducted with a 1:1 molar ratio (CA to metal) at 60-70°C in natural media (pH 7-8), which consisted of a binary solvent of MeOH and H2O (1:1). The resulting CA complexes were characterized using analytical data (metal, H, C, and Cl, analysis) and spectral data (UV-visible, FT-IR, and 1H NMR) to elucidate the complexes’ structures. The results suggested that CA in anion form utilized oxygen atoms of the carboxylate group (-COO−) to capture Zn(II), Mg(II), and Ca(II) ions. This produced complexes with the general compositions of [Zn(CA)(H2O)Cl], [Mg(CA)(H2O)Cl]H2O, and [Ca(CA)(H2O)Cl]2H2O, respectively. The Kirby-Bauer disc diffusion assay was then used to explore the bioactivity of the CA complexes toward three fungal species, three Gram-positive bacteria, and two Gram-negative bacteria. The Ca(II) and Mg(II) complexes exhibited marked inhibitory effects on the cell growth of the fungal species Aspergillus niger with potency equal to 127% and 116% of the activity of the positive control, respectively. The Zn(II) and Ca(II) complexes strongly inhibited the growth of Penicillium sp., while the Zn(II) and Mg(II) complexes showed strong growth inhibition towards the Gram-negative species Pseudomonas aeruginosa.

Keywords

Chenodeoxycholic acid; Metal-based complex; Spectroscopy; Microscopic images; Antimicrobial test

Subject

Chemistry and Materials Science, Applied Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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