Vrkoslav, V.; Smeringaiova, I.; Smorodinova, N.; Svobodova, A.; Strnad, S.; Jackson, C.J.; Burkert, J.; Jirsova, K. Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane. Bioengineering2023, 10, 740.
Vrkoslav, V.; Smeringaiova, I.; Smorodinova, N.; Svobodova, A.; Strnad, S.; Jackson, C.J.; Burkert, J.; Jirsova, K. Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane. Bioengineering 2023, 10, 740.
Vrkoslav, V.; Smeringaiova, I.; Smorodinova, N.; Svobodova, A.; Strnad, S.; Jackson, C.J.; Burkert, J.; Jirsova, K. Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane. Bioengineering2023, 10, 740.
Vrkoslav, V.; Smeringaiova, I.; Smorodinova, N.; Svobodova, A.; Strnad, S.; Jackson, C.J.; Burkert, J.; Jirsova, K. Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane. Bioengineering 2023, 10, 740.
Abstract
To compare concentrations of endogenous N-acylethanolamines (NAEs) lipid mediators pal-mitoylethanolamide (PEA), oleoylethanolamide (OEA), and anandamide (AEA) in fresh, decon-taminated, cryopreserved and freeze-dried amniotic membrane (AM) allografts. Thereby deter-mining whether AM's analgesic and anti-inflammatory efficiency related to NAEs persists during storage. The concentrations of NAEs were measured using ultra-high-performance liquid chro-matography-tandem mass spectrometry. Indirect fluorescent immunohistochemistry was used to detect the PEA PPARα receptor. The concentrations of PEA, OEA, and AEA were significantly higher after decontamination. A significant decrease was found in cryopreserved AM compared to decontaminated tissue for PEA but not for OEA and AEA. However, significantly higher val-ues for all NAEs were detected in cryopreserved samples compared to fresh tissue before decon-tamination. The freeze-dried AM had similar values to decontaminated AM with no statistically significant difference. The nuclear staining of PPARα receptor was clearly visible in all specimens. The stability of NAEs in AM after cryopreservation was demonstrated under tissue bank storage conditions. However, a significant decrease, but still higher concentration of PEA compared to fresh not decontaminated tissue was found in cryopreserved, but not frieze-dried AM. Results in-dicate that NAEs persist during storage in levels sufficient for the analgesic and anti-inflammatory effects of AM allografts.
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