Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Modulation of Cell Response Through the Covalent Binding of Fibronectin to Titanium Substrates

Parsa Rezvanian
ORCID logo ,
Aroa Alvarez-Lopez
ORCID logo ,
Raquel Tabraue
,
Rafael Daza
,
Luis Colchero
,
Manuel Elices
,
Gustavo V. Guinea
,
Daniel González-Nieto
ORCID logo ,
José Pérez-Rigueiro
* ORCID logo
Version 1 : Received: 25 May 2023 / Approved: 26 May 2023 / Online: 26 May 2023 (05:50:27 CEST)

A peer-reviewed article of this Preprint also exists.

Rezvanian, P.; Álvarez-López, A.; Tabraue-Rubio, R.; Daza, R.; Colchero, L.; Elices, M.; Guinea, G.V.; González-Nieto, D.; Pérez-Rigueiro, J. Modulation of Cell Response through the Covalent Binding of Fibronectin to Titanium Substrates. J. Funct. Biomater. 2023, 14, 342. Rezvanian, P.; Álvarez-López, A.; Tabraue-Rubio, R.; Daza, R.; Colchero, L.; Elices, M.; Guinea, G.V.; González-Nieto, D.; Pérez-Rigueiro, J. Modulation of Cell Response through the Covalent Binding of Fibronectin to Titanium Substrates. J. Funct. Biomater. 2023, 14, 342.

Abstract

Titanium (Ti-6Al-4V) substrates were functionalized through the covalent binding of fibronectin, and the effect of the presence of this extracellular matrix protein on the surface of the material was assessed employing mesenchymal stem cell (MSC) cultures. The functionalization process com-prised the usage of the activation vapor silanization (AVS) technique to deposit a thin film with a high surface density of amine groups on the material, followed by the covalent binding of fi-bronectin to the amine groups using the N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hy-drochloride / N-hydroxysuccinimide (EDC/NHS) crosslinking chemistry. The biological effect of the fibronectin on murine MSCs was assessed in vitro. It was found that functionalized samples not only showed enhanced initial cell adhesion compared with bare titanium, but also a three-fold increase in the cell area, reaching values comparable to those found on the polystyrene controls. These results represent a clear indication of the potential of modulating the response of the or-ganism to an implant through the covalent binding of extracellular matrix proteins on the pros-thesis.

Keywords

fibronectin; functionalization; activated vapor silanization (AVS); mesenchymal stem cells (MSC); biomaterial.

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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