Kubo, A.; Matsubara, K.; Matsubara, Y.; Nakaoka, H.; Sugiyama, T. The Influence of Nicotine on Trophoblast-Derived Exosomes in a Mouse Model of Pathogenic Preeclampsia. Int. J. Mol. Sci.2023, 24, 11126.
Kubo, A.; Matsubara, K.; Matsubara, Y.; Nakaoka, H.; Sugiyama, T. The Influence of Nicotine on Trophoblast-Derived Exosomes in a Mouse Model of Pathogenic Preeclampsia. Int. J. Mol. Sci. 2023, 24, 11126.
Kubo, A.; Matsubara, K.; Matsubara, Y.; Nakaoka, H.; Sugiyama, T. The Influence of Nicotine on Trophoblast-Derived Exosomes in a Mouse Model of Pathogenic Preeclampsia. Int. J. Mol. Sci.2023, 24, 11126.
Kubo, A.; Matsubara, K.; Matsubara, Y.; Nakaoka, H.; Sugiyama, T. The Influence of Nicotine on Trophoblast-Derived Exosomes in a Mouse Model of Pathogenic Preeclampsia. Int. J. Mol. Sci. 2023, 24, 11126.
Abstract
Preeclampsia (PE) is a serious complication of pregnancy with a pathogenesis that is not fully understood, though it involves the impaired invasion of extravillous trophoblasts (EVTs) into the decidual layer during implantation. Recently, others have found that diverse cell types, including EVTs, produce exosomes filled with molecular cargo (mainly proteins and RNAs) that can be transported to other cells and organs both locally and long-range. The cargo delivered by exosomes can signal to and modify the receiving cells and their environment, and EVT-derived exosomes may influence the pathogenesis of PE. Because the risk of PE is actually decreased by cigarette smoking, we considered the possibility that nicotine, a critical component of tobacco smoke, might protect against PE by modifying the content of exosomes from EVTs. In this study, we applied nicotine stimulation to cultured EVTs and subjected their secreted exosomes to proteomic analysis. We identified many proteins whose abundance in exosomes was modified by nicotine treatment of the donor EVTs, and we used bioinformatic annotation and network analysis to select five key hub proteins with potential roles in the pathogenesis or prevention of PE.
Medicine and Pharmacology, Obstetrics and Gynaecology
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