Rodríguez-Torres, I.; Martín Carrillo, A.; Ramírez, M.P.; Gómez Gálvez, F.J.; Velasco Arjona, L.; Padilla, C.; Cretazzo, E. Genotypic and Sanitary Characterization of Minority Grapevine Varieties Prospected in Andalusia, Spain. Horticulturae2023, 9, 759.
Rodríguez-Torres, I.; Martín Carrillo, A.; Ramírez, M.P.; Gómez Gálvez, F.J.; Velasco Arjona, L.; Padilla, C.; Cretazzo, E. Genotypic and Sanitary Characterization of Minority Grapevine Varieties Prospected in Andalusia, Spain. Horticulturae 2023, 9, 759.
Rodríguez-Torres, I.; Martín Carrillo, A.; Ramírez, M.P.; Gómez Gálvez, F.J.; Velasco Arjona, L.; Padilla, C.; Cretazzo, E. Genotypic and Sanitary Characterization of Minority Grapevine Varieties Prospected in Andalusia, Spain. Horticulturae2023, 9, 759.
Rodríguez-Torres, I.; Martín Carrillo, A.; Ramírez, M.P.; Gómez Gálvez, F.J.; Velasco Arjona, L.; Padilla, C.; Cretazzo, E. Genotypic and Sanitary Characterization of Minority Grapevine Varieties Prospected in Andalusia, Spain. Horticulturae 2023, 9, 759.
Abstract
Andalusia is a Spanish region that is home to numerous minority varieties due to its diversity and territorial extension, offering the local viticulture the possibility of diversifying its wine production. The molecular identification of 98 specimens from six areas with a winemaking tradition in Andalusia was carried out between the years 2020 and 2022. Thirteen microsatellite markers were used in this study, including the nine recommended by the OIV. 31 different genotypes were obtained, 20 of which corresponded to profiles of already described varieties (11 of them are of minority cultivation in Andalusia: 'Rojal Tinto', 'Beba', 'Zurieles', 'Rome', 'Hebén', 'Mollar Cano', 'Listán Prieto', 'Listán del Condado', 'Jarrosuelto', 'Negra Dorada' and 'Mantúo de Pilas'), while the other 11 profiles did not match with previously identified varieties. This profiles were integrated into the database of the IFAPA “Rancho de la Merced” Germplasm Bank. The eco-geographical groups of the new identified genotypes were determined through analysis of genetic diversity. The presence of the Grapevine Fanleaf Virus, the Grapevine Fleck Virus and the Grapevine Leafroll associated Viruses was also analyzed using an ELISA test due to the necessity of having certifiable clones of the new varieties for their potential interest in being authorized for its cultivation in Spain.
Keywords
genetic resources; molecular markers; local cultivar; certification
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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