Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Study on Cubic-Orientation Settlement of Angiotensin Converting Enzyme II Immobilized on Polystyrene by Molecular Dynamics Simulation

Version 1 : Received: 18 May 2023 / Approved: 19 May 2023 / Online: 19 May 2023 (10:20:13 CEST)

How to cite: Ma, Y.; Yuan, Y.J. Study on Cubic-Orientation Settlement of Angiotensin Converting Enzyme II Immobilized on Polystyrene by Molecular Dynamics Simulation. Preprints 2023, 2023051428. https://doi.org/10.20944/preprints202305.1428.v1 Ma, Y.; Yuan, Y.J. Study on Cubic-Orientation Settlement of Angiotensin Converting Enzyme II Immobilized on Polystyrene by Molecular Dynamics Simulation. Preprints 2023, 2023051428. https://doi.org/10.20944/preprints202305.1428.v1

Abstract

The adsorption of proteins on polymer is widely used in biosensors. Here, molecular dynamics (MD) simulation was used to study the immobilization of angiotensin converting enzyme II (ACE2) with six initial orientations proposed on polystyrene (PS) at the ambient conditions of pH (4.5, 6, 7, 8, 9.5) and NaCl (0.01, 0.05, 0.1, 0.15, 0.2, 0.25 M). ACE2 immobilization under favorable ambient conditions was characterized by minimum distance (short), settlement time (fast), interaction energy (substantial) and protein configuration (stable). ACE2 orientations proposed in 0.15M NaCl were respectively preferable to (90, 0, 0), (0, 0, 0) and (0, 270, 0), (180, 0, 0) and (0, 90, 0), (90, 0, 0) at pH 4.5, 6, 7, 9.5. ACE2 immobilization was further evaluated at pH 7 by optimizing NaCl concentration. Its proposed orientations of (i) (0, 270, 0), (ii) (0, 0, 0) and (90, 0, 0), and (iii) (0, 90, 0) and (90, 0, 0) were preferable in 0.05, 0.1 and 0.2 M NaCl, respectively. The great significance of cubic-orientation settlement mode provides tangible improvement for the microfabrication of biochips for rapid diagnosis of severe acute respiratory syndrome coronavirus II (SARS-CoV-2).

Keywords

Angiotensin converting enzyme II; Polystyrene; Molecular dynamics simulation; Adsorption behaviors; Biochips

Subject

Chemistry and Materials Science, Biomaterials

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