Zhao, Z.; Yang, D.; Guo, J.; Liu, X.; Li, Q.; Su, P.; Wang, J.; Ma, Y.; Huang, L. Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants. Molecules2023, 28, 4711.
Zhao, Z.; Yang, D.; Guo, J.; Liu, X.; Li, Q.; Su, P.; Wang, J.; Ma, Y.; Huang, L. Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants. Molecules 2023, 28, 4711.
Zhao, Z.; Yang, D.; Guo, J.; Liu, X.; Li, Q.; Su, P.; Wang, J.; Ma, Y.; Huang, L. Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants. Molecules2023, 28, 4711.
Zhao, Z.; Yang, D.; Guo, J.; Liu, X.; Li, Q.; Su, P.; Wang, J.; Ma, Y.; Huang, L. Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants. Molecules 2023, 28, 4711.
Abstract
Salvia is a large genus in traditional Chinese medicine with hundreds of species. Tanshinones are a highly representative class of exclusive compounds found in the Salvia genus that exhibit significant biological activity. Tanshinone components have been identified in 16 species of the Salvia genus. The CYP76AH subfamily (P450) is crucial for the synthesis of tanshinone due to its catalytic generation of polyhydroxy structures. 420CYP76AH genes were obtained, clear clustering relationships. In this study 15 CYP76AH genes from 10 Salvia species were cloned and studied from the perspectives of evolution and catalytic efficiency. Structure-function relationship revealed several conserved residues which might be related to the function of CYP76AHs. Amino acids (97I, 116V, and 290T) are conserved in CYP76AH1 group, while amino acids (117G, 208S, 237Y, and 479F) are CYP76AH3 group protein sequence characteristics with unique enzyme function.This research holds significant implications for the classification of the Salvia genus and provide efficient catalytic elements for the synthetic biology production of tanshinones.Keywords: Salvia; CYP76AH; P450; biosynthesis; functional gene
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