Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Whole Yeast Vaccine Displaying ZIKV B and T Cell Epitopes Induces Humoral and Cellular Immune Responses in Murine Model

Anna Jéssica Duarte Silva
ORCID logo ,
André Luiz Santos de Jesus
,
Lígia Rosa Sales Leal
ORCID logo ,
Larissa Silva Macêdo
ORCID logo ,
Bárbara Rafaela da Silva Barros
,
Georon Ferreira De Sousa
ORCID logo ,
Simone da Paz Leôncio Alves
,
Lindomar José Pena
,
Cristiane Moutinho Lagos De Melo
ORCID logo ,
Antonio Carlos de Freitas
* ORCID logo
Version 1 : Received: 15 May 2023 / Approved: 17 May 2023 / Online: 17 May 2023 (11:00:56 CEST)

A peer-reviewed article of this Preprint also exists.

Silva, A.J.D.; de Jesus, A.L.S.; Leal, L.R.S.; de Macêdo, L.S.; da Silva Barros, B.R.; de Sousa, G.F.; da Paz Leôncio Alves, S.; Pena, L.J.; de Melo, C.M.L.; de Freitas, A.C. Whole Yeast Vaccine Displaying ZIKV B and T Cell Epitopes Induces Cellular Immune Responses in the Murine Model. Pharmaceutics 2023, 15, 1898. Silva, A.J.D.; de Jesus, A.L.S.; Leal, L.R.S.; de Macêdo, L.S.; da Silva Barros, B.R.; de Sousa, G.F.; da Paz Leôncio Alves, S.; Pena, L.J.; de Melo, C.M.L.; de Freitas, A.C. Whole Yeast Vaccine Displaying ZIKV B and T Cell Epitopes Induces Cellular Immune Responses in the Murine Model. Pharmaceutics 2023, 15, 1898.

Abstract

Improving antigen presentation is crucial for the success of immunization strategies. Yeasts are classically used as biofactories to produce recombinant proteins and are efficient vehicles for the delivery of vaccine antigens, besides present adjuvants properties. Despite the absence of epidemic outbreaks, several vaccine approaches continue to be developed for Zika virus infection. These prophylactic strategies are fundamental given the severity of clinical manifestations, mainly due to viral neurotropism. The present study aimed to evaluate in vivo the immune response induced by P. pastoris recombinant strains displaying epitopes of the Envelope (ENV) and NS1 ZIKV proteins. Intramuscular immunization with heat-attenuated yeast enhanced the secretion of IL-6, TNF-α, and IFN-γ, besides activation of CD4+ and CD8+ T cells, in BALB/c mice. P. pastoris displaying ENV epitopes induced a more robust immune response, increasing immunoglobulin production, especially IgG isotypes. Both proposed vaccines showed the potential to induce immune responses without adverse effects, confirming the safety of administering P. pastoris as a vaccine vehicle. Here we demonstrated, for the first time, the evaluation of a vaccine against ZIKV based on a multiepitope construct, using yeast as a vehicle, reinforcing the applicability of P. pastoris as a whole yeast cell vaccine.

Keywords

Vaccine; Antigen-delivery; ZIKV; Yeast

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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