Chen, Y.; Liu, H.; Tang, Y.; Zhang, L.; Wu, X.J.; Guo, Q.Q.; Ding, Y.; Wei, M.C.; Shen, X.C.; Tao, L. Construction of Different Oil Phases-Based Nanoemulsion Delivery Platform and Biodistribution Evaluation on Inflammatory Cells and Animal Models. Preprints2023, 2023051164. https://doi.org/10.20944/preprints202305.1164.v1
APA Style
Chen, Y., Liu, H., Tang, Y., Zhang, L., Wu, X.J., Guo, Q.Q., Ding, Y., Wei, M.C., Shen, X.C., & Tao, L. (2023). Construction of Different Oil Phases-Based Nanoemulsion Delivery Platform and Biodistribution Evaluation on Inflammatory Cells and Animal Models. Preprints. https://doi.org/10.20944/preprints202305.1164.v1
Chicago/Turabian Style
Chen, Y., Xiang Chun Shen and Ling Tao. 2023 "Construction of Different Oil Phases-Based Nanoemulsion Delivery Platform and Biodistribution Evaluation on Inflammatory Cells and Animal Models" Preprints. https://doi.org/10.20944/preprints202305.1164.v1
Abstract
Inflammation is the response of the immune system to infection and injury, with complex interactions with the body, tissues and cells, and plays an important role in the initiation and progression of many diseases such as cancer and cardiovascular disease. Traditional excipients functioning as drug delivery systems can reduce the side effects of systemic distribution of inflammatory drugs. Oil phase, a common excipient in nanoemulsions (NEs), has the advantages of assisting the formation of emulsions, loading lipophilic drugs, thermodynamic stability, wide sources, low price and easy availability. In particular, some of them, like linolenic acid and medium chain triglyceride (MCT), also function as inhibitors of systemic inflammation marker and vascular inflammation marker. However, the influences of different oil phases on the construction of NEs and their distribution in vivo have not been fully investigated. Herein, Tween 80 and ethyl alcohol were screened out as emulsifiers and co-emulsifiers, and three different oil phases used in clinical practice widely, including MCT, oleic acid (OA), and ethyl oleate (EO), were selected as the oil phase to prepare NEs delivery platform. Three NEs had consistent size, potential, morphology, excellent stability and mitochondrial localization function. Importantly, they exhibited superior cellular uptake capacity in inflammatory-injuried human umbilical vein endothelial cells and significantly targeted the brain, lung, heart and thoracic aorta in the lipopolysaccharide-induced mouse and zebrafish larvae inflammation models. Meanwhile, the fluorescence intensities of three NEs were different in these organs, suggesting that their differential enrichment might provide alternative delivery platform for disease treatment in the corresponding organs.
Keywords
Inflammation; excipients; oil phases; nanoemulsion; distribution
Subject
Medicine and Pharmacology, Pharmacy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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