Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of the In Vitro Antimicrobial Efficacy Against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/ Chitosan/ Vancomycin. Preclinical Study

Iván López-González
,
Ana Belén Hernández-Heredia
ORCID logo ,
María Isabel Rodríguez-López
ORCID logo ,
David Auñón-Calles
,
Mohamed Boudifa
,
José Antonio Gabaldón
* ORCID logo ,
Luis Meseguer-Olmo
*
Version 1 : Received: 8 May 2023 / Approved: 9 May 2023 / Online: 9 May 2023 (12:05:37 CEST)

A peer-reviewed article of this Preprint also exists.

López-González, I.; Hernández-Heredia, A.B.; Rodríguez-López, M.I.; Auñón-Calles, D.; Boudifa, M.; Gabaldón, J.A.; Meseguer-Olmo, L. Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study. Pharmaceutics 2023, 15, 1763. López-González, I.; Hernández-Heredia, A.B.; Rodríguez-López, M.I.; Auñón-Calles, D.; Boudifa, M.; Gabaldón, J.A.; Meseguer-Olmo, L. Evaluation of the In Vitro Antimicrobial Efficacy against Staphylococcus aureus and epidermidis of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study. Pharmaceutics 2023, 15, 1763.

Abstract

Acute and chronic bone infections, especially those caused by methicillin-resistant Staphylococcus aureus (MRSA), remains a major complication and therapeutic challenge. It is documented that local administration of vancomycin offers better results than the usual routes of administration (e.g. intravenous) when ischemic areas are present. In this work, we evaluate the antimicrobial efficacy against S. aureus and S. epidermidis and analyzed the potential cytotoxic effect of a novel hybrid 3D-printed scaffold based on poly(caprolactone) and chitosan loaded with different vancomycin concentrations (1, 5, 10 and 20%). For this purpose, vancomycin release was measured by means of HPLC, and the biological response of ah-BM-MSCs in the presence of the scaffolds was evaluated in terms of cytotoxicity (LDH activity), proliferation (AlamarBlueⓇ) and osteogenic differentiation (ALP activity, Alizarin Red staining). In addition, two cold plasma treatments were evaluated to improve the adhesion of hydrophobic polymers to hydrogels. The hybrid PCL/CS/Van scaffolds tested were found to be biocompatible, bioactive, and bactericide, as demonstrated by no cytotoxicity or functional alteration and by bacterial inhibition. Our results suggest that the scaffolds developed would be excellent candidates to be used in a wide range of biomedical fields such as drug delivery systems or tissue engineering applications.

Keywords

3D printing; hybrid scaffold; poly(caprolactone); chitosan; vancomycin; mesenchymal stem cells; tissue engineering; drug-delivery systems (DDSs); M osteomyelitis

Subject

Medicine and Pharmacology, Medicine and Pharmacology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.