Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Impact on Kidney Transplantation of the Percentage of T Lymphocytes Isolated from Hepatic Perfusate of Multi-Organ Deceased Brain Donors

Duilio Pagano
ORCID logo ,
Ester Badami
ORCID logo ,
Giovanni Zito
ORCID logo ,
Pier Giulio Conaldi
ORCID logo ,
Ivan Vella
,
Barbara Buscemi
,
Giandomenico Amico
ORCID logo ,
Rosalia Busà
,
Paola Salis
,
Sergio Li Petri
,
Fabrizio Di Francesco
,
Sergio Calamia
,
Pasquale Bonsignore
,
Alessandro Tropea
,
Caterina Accardo
,
Salvatore Piazza
,
Salvatore Gruttadauria
* ORCID logo
Version 1 : Received: 6 May 2023 / Approved: 8 May 2023 / Online: 8 May 2023 (05:16:31 CEST)

A peer-reviewed article of this Preprint also exists.

Pagano, D.; Badami, E.; Zito, G.; Conaldi, P.G.; Vella, I.; Buscemi, B.; Amico, G.; Busà, R.; Salis, P.; Li Petri, S.; di Francesco, F.; Calamia, S.; Bonsignore, P.; Tropea, A.; Accardo, C.; Piazza, S.; Gruttadauria, S. Impact of T Lymphocytes Isolated from Liver Perfusate of Deceased Brain Donors on Kidney Transplantation: Preliminary Evidence and Future Directions. J. Clin. Med. 2023, 12, 4786. Pagano, D.; Badami, E.; Zito, G.; Conaldi, P.G.; Vella, I.; Buscemi, B.; Amico, G.; Busà, R.; Salis, P.; Li Petri, S.; di Francesco, F.; Calamia, S.; Bonsignore, P.; Tropea, A.; Accardo, C.; Piazza, S.; Gruttadauria, S. Impact of T Lymphocytes Isolated from Liver Perfusate of Deceased Brain Donors on Kidney Transplantation: Preliminary Evidence and Future Directions. J. Clin. Med. 2023, 12, 4786.

Abstract

Background: Hepatic interstitial T-lymphocytes (T-Li), Natural Killer (NK) and NK-T cells play an important role in both innate and adaptive immunity and contribute to the regulation of ischemia/reperfusion injury (IRI) after transplantation of abdominal organ. Methods: The cellular concentrations and phenotypes of NK, T-Li, NK-T were analyzed retrospectively in a consecutive series of hepatic perfusates after surgical removal of whole livers on the bench previously collected from adult multi-organ donors after brain death. (DBD), and compared with the demographic and pathological characteristics of the patients transplanted at our Institute with kidneys taken from the same donors. The liver interstitial cells were purified from the perfusate by density gradient centrifugation and the phenotype was determined by flow cytometric investigation using the following immunological markers: CD3, CD4, CD8 and CD56 in order to determine the relative percentage of T-Li, NK-T and NK cells. Results: The perfusates of 42 DBDs, and the related clinical outcome of kidney transplant recipients from 2010 to 2020, were analyzed. T-Li were significantly associated with the time in days of delayed functional recovery of transplanted kidneys (DGF) (p = 0.02), to onset of secondary infection from Cytomegalovirus (p = 0.03). On COX analysis, percent cell concentration of T-Li and time to DGF were significantly associated with an increased relative risk (HR) of organ survival (HR = 1.038, p = 0.04; and HR = 1.029, p = 0.01, respectively). The specificity of the NK and NK-T cell proportions were not associated with any relevant clinical outcomes in kidney transplant patients. Conclusions: The present study points to a new potential role of T-Li cells detected in the context of liver perfusate DBD, and could detect potential impacts in organ allocation, surgical harvesting techniques and in the analysis of IRI pathophysiological events after kidney transplants from multi-organ DBDs.

Keywords

: Kidney transplantation; hepatic perfusate; deceased brain donors; T lymphocytes

Subject

Medicine and Pharmacology, Transplantation

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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