Corcoll, F.; Pérez-Prieto, D.; Karbysheva, S.; Trampuz, A.; Fariñas, O.; Monllau, J.C. Are Hamstring Grafts a Predisposing Factor to Infection in R-ACL Surgery? A Comparative In Vitro Study. Pathogens2023, 12, 761.
Corcoll, F.; Pérez-Prieto, D.; Karbysheva, S.; Trampuz, A.; Fariñas, O.; Monllau, J.C. Are Hamstring Grafts a Predisposing Factor to Infection in R-ACL Surgery? A Comparative In Vitro Study. Pathogens 2023, 12, 761.
Corcoll, F.; Pérez-Prieto, D.; Karbysheva, S.; Trampuz, A.; Fariñas, O.; Monllau, J.C. Are Hamstring Grafts a Predisposing Factor to Infection in R-ACL Surgery? A Comparative In Vitro Study. Pathogens2023, 12, 761.
Corcoll, F.; Pérez-Prieto, D.; Karbysheva, S.; Trampuz, A.; Fariñas, O.; Monllau, J.C. Are Hamstring Grafts a Predisposing Factor to Infection in R-ACL Surgery? A Comparative In Vitro Study. Pathogens 2023, 12, 761.
Abstract
Background: The objective of the present study was to evaluate the formation of biofilm in bone patellar tendon bone grafts (BPTB grafts) and to compare it to the formation of biofilm formation in quadrupled hamstring anterior cruciate ligament grafts (4xHt graft). Methods: A descriptive in vitro study was conducted. One 4xHt graft and one BPTB graft were prepared. They were then contaminated with a strain of S. epidermidis. Later, a quantitative analysis was carried out by means of microcalorimetry and sonication with plating. Additionally, a qualitative analysis was carried out by means of electron microscopy. Results: No significant differences were found between the bacterial growth profiles of 4xHt graft and BPTB graft in microcalorimetry and colony counting. In the samples analyzed with electron microscopy, no specific biofilm growth pattern was identified upon comparing BPTB graft to 4xHt graft. Conclusions: There were no significant differences at either the quantitative or qualitative level when comparing bacterial growth in BPTB graft to that in 4xHt graft. Therefore, the presence of sutures in 4xHt graft cannot be established as a predisposing factor for increased biofilm growth in this in vitro study.
Medicine and Pharmacology, Orthopedics and Sports Medicine
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