Version 1
: Received: 4 May 2023 / Approved: 4 May 2023 / Online: 4 May 2023 (08:57:52 CEST)
How to cite:
Mallonga, Z.; Filgueira, L. An In vitro Model of Chlamydia trachomatis Infection in Human Dendritic Cells. Preprints2023, 2023050242. https://doi.org/10.20944/preprints202305.0242.v1
Mallonga, Z.; Filgueira, L. An In vitro Model of Chlamydia trachomatis Infection in Human Dendritic Cells. Preprints 2023, 2023050242. https://doi.org/10.20944/preprints202305.0242.v1
Mallonga, Z.; Filgueira, L. An In vitro Model of Chlamydia trachomatis Infection in Human Dendritic Cells. Preprints2023, 2023050242. https://doi.org/10.20944/preprints202305.0242.v1
APA Style
Mallonga, Z., & Filgueira, L. (2023). An In vitro Model of <em>Chlamydia trachomatis</em> Infection in Human Dendritic Cells. Preprints. https://doi.org/10.20944/preprints202305.0242.v1
Chicago/Turabian Style
Mallonga, Z. and Luis Filgueira. 2023 "An In vitro Model of <em>Chlamydia trachomatis</em> Infection in Human Dendritic Cells" Preprints. https://doi.org/10.20944/preprints202305.0242.v1
Abstract
Chlamydia trachomatis (CT) is an obligate intracellular bacterial pathogen known to cause trachoma, sexually transmitted infections, and reactive arthritis. The more serious sequelae of these diseases, blindness from trachoma and pelvic inflammatory disease from chlamydial STD are immunopathological responses to chronic infections. With the abundance of dendritic cells in CT infection sites and their central importance in immune regulation, the susceptibility of dendritic cells to CT infection was investigated in vitro. CT (serovar F) was grown and extracted from HeLa 229 cells for infection of human dendritic cells (DCs). Human DCs were generated from peripheral blood mononuclear cells in the presence of GM-CSF and IL-4. Infected DCs were harvested at 0, 4, 24, and 48 h after infection and immunostained. Results from fluorescence microscopy showed that DC can be infected and can support the growth of CT in vitro. Intracellular elementary and reticulate bodies of CT were detected, and reticulate bodies were shown to multiply over the 48 h-cycle in DC. At 48 h, intracellular elementary bodies were detected in some DCs. These results may provide new insights into the possible participation of DC in sustaining chronic or latent infection, or even dissemination of CT infection to other sites.
Keywords
Chlamydia trachomatis; elementary bodies; human dendritic cells; in vitro; reticulate bodies
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
