Version 1
: Received: 28 April 2023 / Approved: 4 May 2023 / Online: 4 May 2023 (02:48:03 CEST)
How to cite:
Hassan, Z.; Philip, P.S.; Baby, B.; Akhtar, M.K.; Shmygol, A.; Khan, G. The Interaction of Ribosomal Protein L22 with Epstein-Barr Virus-Encoded RNA-1 in Intracellular Transport. Preprints2023, 2023050163. https://doi.org/10.20944/preprints202305.0163.v1
Hassan, Z.; Philip, P.S.; Baby, B.; Akhtar, M.K.; Shmygol, A.; Khan, G. The Interaction of Ribosomal Protein L22 with Epstein-Barr Virus-Encoded RNA-1 in Intracellular Transport. Preprints 2023, 2023050163. https://doi.org/10.20944/preprints202305.0163.v1
Hassan, Z.; Philip, P.S.; Baby, B.; Akhtar, M.K.; Shmygol, A.; Khan, G. The Interaction of Ribosomal Protein L22 with Epstein-Barr Virus-Encoded RNA-1 in Intracellular Transport. Preprints2023, 2023050163. https://doi.org/10.20944/preprints202305.0163.v1
APA Style
Hassan, Z., Philip, P.S., Baby, B., Akhtar, M.K., Shmygol, A., & Khan, G. (2023). The Interaction of Ribosomal Protein L22 with Epstein-Barr Virus-Encoded RNA-1 in Intracellular Transport. Preprints. https://doi.org/10.20944/preprints202305.0163.v1
Chicago/Turabian Style
Hassan, Z., Anatoliy Shmygol and Gulfaraz Khan. 2023 "The Interaction of Ribosomal Protein L22 with Epstein-Barr Virus-Encoded RNA-1 in Intracellular Transport" Preprints. https://doi.org/10.20944/preprints202305.0163.v1
Abstract
Epstein-Barr virus (EBV)-encoded RNAs (EBERs) are structurally conserved small, noncoding RNAs constitutively expressed in EBV infection. Although primarily localised in the nucleus, EBERs are found in the cytoplasm and exosomes. However, the mechanism(s) of EBERs transport is not known. This study aimed to investigate the structural impact of EBER1 on its transport. EBER1 stem-loop (SL) deletion mutants (ΔSL1, ΔSL3 and ΔSL4) were created and stably transfected into HEK293T cells. The expression of EBER1 was quantified in total cell, nuclear, cytoplasmic and exosomal fractions. The quantification was performed in the presence of physiological expression of RPL22 and La, and after silencing them. These proteins are believed to be involved in EBER1 transport and secretion. Compared to the wildtype EBER1 transfectants, the expression level of EBER1 gene in all mutants was significantly lower in the total cell, cytoplasmic and exosomal fractions. However, ΔSL3 mutant showed significant nuclear retention. Silencing RPL22 resulted in increased nuclear-cytoplasmic trafficking of EBER1. Silencing La protein did not affect EBER1 secretion. Alternatively, the store-operated intracellular Ca2+ was found to correlate with EBER1 expression in exosomes. Taken together, EBER1 structure and its interaction with RPL22 appeared to be important in the nuclear-cytoplasmic transport of the RNA.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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