Addisu, A.; Dogan, Y.E.; Montgomery, D.; Addisu, L.; Alli, A.A. Non-muscle Myosin Type-II Localization in the Mouse Intestinal Villi is Altered by B-Type Natriuretic Peptide. Preprints2023, 2023050012. https://doi.org/10.20944/preprints202305.0012.v1
APA Style
Addisu, A., Dogan, Y.E., Montgomery, D., Addisu, L., & Alli, A.A. (2023). Non-muscle Myosin Type-II Localization in the Mouse Intestinal Villi is Altered by B-Type Natriuretic Peptide. Preprints. https://doi.org/10.20944/preprints202305.0012.v1
Chicago/Turabian Style
Addisu, A., Leeyu Addisu and Abdel A Alli. 2023 "Non-muscle Myosin Type-II Localization in the Mouse Intestinal Villi is Altered by B-Type Natriuretic Peptide" Preprints. https://doi.org/10.20944/preprints202305.0012.v1
Abstract
Our prior studies have shown that B type Natriuretic Peptide (BNP) decreases absorption from the gastrointestinal (GI) tract. Since non-muscle myosin-type II (NMM-II) is known to have a role in epithelial tight junction regulation, we aimed to test whether BNP has an effect on the localization and expression of NMM-II in the intestinal villi. We treated wild type mice with a 10 ng/g intravenous bolus of BNP followed by infusion of BNP at 1ng/g/minute vs. vehicle control. The mice were then euthanized and intestinal tissue isolated and sectioned. The tissue was immune-stained for NMM-II and examined using fluorescence microscopy. The tissue section not treated with antibody to NMM-II was used as a methodological control to evaluate the non-specific binding. Scanning electron micrographic images were taken to compare structural differences between the two groups. Western blotting was performed to compare the regional protein expression of NMM-II and associated proteins including smooth muscle actin, kinesin, and E cadherin in small intestinal tissue between BNP vs. vehicle infused mice. Fluorescence microscopy revealed markedly increased localization of NMM-II at the crypts and core of intestinal villi of the jejunum, ileum and colon of BNP treated mice compared to vehicle. Transmission electron microscopy revealed that the microvilli of BNP treated mice assumed the distinctive appearance of ‘relaxed’ microvilli compared to vehicle. Such decrease in contractility was previously shown to decrease para-cellular permeability of epithelia. Our observation indicates that BNP alters the localization of NMM-II in intestinal villi and makes the intestinal villi structures assume a state of decreased permeability of the apical junctional complex. Further characterization of this process and understanding the specific mediators involved could lead to the identification of novel therapeutic targets for various disease states such as heart failure and malabsorption.
Keywords
Non-Muscle Myosin; BNP; GI epithelia; Apical junctional complex; Absorption
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
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