Version 1
: Received: 26 April 2023 / Approved: 27 April 2023 / Online: 27 April 2023 (03:41:10 CEST)
How to cite:
Hage, W.; Karam Sarkis, D.; Kallassy, M.; Sfeir, G.; Mallah, M.; Khalil, I.; Zogheib, C. Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers Against a Multispecies Biofilm. Preprints2023, 2023041015. https://doi.org/10.20944/preprints202304.1015.v1
Hage, W.; Karam Sarkis, D.; Kallassy, M.; Sfeir, G.; Mallah, M.; Khalil, I.; Zogheib, C. Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers Against a Multispecies Biofilm. Preprints 2023, 2023041015. https://doi.org/10.20944/preprints202304.1015.v1
Hage, W.; Karam Sarkis, D.; Kallassy, M.; Sfeir, G.; Mallah, M.; Khalil, I.; Zogheib, C. Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers Against a Multispecies Biofilm. Preprints2023, 2023041015. https://doi.org/10.20944/preprints202304.1015.v1
APA Style
Hage, W., Karam Sarkis, D., Kallassy, M., Sfeir, G., Mallah, M., Khalil, I., & Zogheib, C. (2023). Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers Against a Multispecies Biofilm. Preprints. https://doi.org/10.20944/preprints202304.1015.v1
Chicago/Turabian Style
Hage, W., Issam Khalil and Carla Zogheib. 2023 "Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers Against a Multispecies Biofilm" Preprints. https://doi.org/10.20944/preprints202304.1015.v1
Abstract
Even after debridement and chemical root canal preparation, multispecies bacterial biofilms can still be found in root canals and dentinal tubules. This demonstrates the necessity of using suitable antibacterial endodontic sealers. Due to their well-known biological qualities, calcium silicate based root canal sealers or “bioceramic’ sealers have recently been launched and can be successful in endodontic procedures. This study's objective was to compare the impact of CerasealR, Total Fill BC SealerR, Bio-C SealerR, AH Plus BioceramicR, K-biocerR and SealiteR on the elimination of a multispecies’ endodontic biofilm at 3, 7 and 14 days.
Enterococcus faecalis, Pseudomonas Aeruginosa, Candida Albicans and Proteus Mirabilis were cultured on specific agars and later grown in Brain Heart Infusion broth on hydroxyapatite discs pretreated with collagen type 1 and incubated for 18 days. The resulting biofilm was then placed on a 10mm round shaped blotting paper set on a metallic net in a 6cm petri dish with an orthodontic bend filled with the calcium silicate based root canal sealer in direct contact with the biofilm.
In all tested groups, the total bacterial count has significantly decreased between day 3 and day 14 (P<0.05) with no statistically significant differences among the different sealers’ groups at all time points. Sealite group was capable of decreasing the Candida Albicans count significantly between day 3 and day 14 (P<0.05) in comparison with the bioceramic groups.
All sealers had antibacterial activity against the multispecies biofilm between day 3 and day 14.
Keywords
bioceramics; multispecies’ biofilm; calcium silicate based root canal sealers; biofilm
Subject
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
,
,
,
