Zhao, D.; Gao, Q.; Zheng, X.; Liu, S.; Qi, Q.; Wang, X.; Yang, X. Optimization of Fermentation Conditions for Elevating Limonene Production with Engineered Rhodosporidium toruloides. Fermentation2023, 9, 431.
Zhao, D.; Gao, Q.; Zheng, X.; Liu, S.; Qi, Q.; Wang, X.; Yang, X. Optimization of Fermentation Conditions for Elevating Limonene Production with Engineered Rhodosporidium toruloides. Fermentation 2023, 9, 431.
Zhao, D.; Gao, Q.; Zheng, X.; Liu, S.; Qi, Q.; Wang, X.; Yang, X. Optimization of Fermentation Conditions for Elevating Limonene Production with Engineered Rhodosporidium toruloides. Fermentation2023, 9, 431.
Zhao, D.; Gao, Q.; Zheng, X.; Liu, S.; Qi, Q.; Wang, X.; Yang, X. Optimization of Fermentation Conditions for Elevating Limonene Production with Engineered Rhodosporidium toruloides. Fermentation 2023, 9, 431.
Abstract
Limonene is a valuable monoterpenoid that with diverse applications. Microbial biosynthesis offers a sustainable alternative to plant-based extraction, which is prone to hinderance by climate change and acreage. In the present study, the effects on limonene production of carbon source, inoculum, working volume, temperature, pH, dodecane coverage, and media were investigated with engineered strains of Rhodosporidium toruloides. Through single-factor and orthogonal experiments, the limonene titer was improved from 52.2 to 358.1 mg/L on 250-mL flask, a 586% increase. The optimized medium was MM, with a working volume of 50 mL, inoculum OD600=0.6, 22 °C, pH 6, and dodecane coverage (v/v) of 20%. The results here demonstrated that the R. toruloides could be explored as an efficient limonene producer and facilitate other monoterpenoids biosynthesis with R. toruloides.
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