Article
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Sample Delivery Systems for Serial Femtosecond Crystallography at PAL-XFEL
Version 1
: Received: 18 April 2023 / Approved: 19 April 2023 / Online: 19 April 2023 (03:22:22 CEST)
A peer-reviewed article of this Preprint also exists.
Park, J.; Nam, K.H. Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL. Photonics 2023, 10, 557. Park, J.; Nam, K.H. Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL. Photonics 2023, 10, 557.
Abstract
Serial femtosecond crystallography (SFX) using an X-ray free-electron laser (XFEL) enables determination of room-temperature structures without causing radiation damage. Using an optical pump-probe or mix-and-injection, SFX enables the intermediate state visualization of a molecular reaction. In SFX experiments, serial and stable microcrystal delivery to the X-ray interaction point is vital for reasonable data collection and efficient beam time. The Pohang Accelerator Laboratory X-ray Free Electron Laser (PAL-XFEL) facility established SFX instruments at a nanocrystallography and coherent imaging (NCI) experimental station. Various sample delivery methods, including injection, fixed-target scanning, and hybrid methods, have been developed and applied to collect XFEL diffraction data. Herein, we review the currently available sample delivery methods for SFX at the NCI experimental station at the PAL-XFEL. This article will help PAL-XFEL users access the SFX system for their experiments.
Keywords
serial femtosecond crystallography; X-ray free electron laser; sample delivery; injector; fixed-target scanning; hybrid method
Subject
Biology and Life Sciences, Biophysics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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