Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Analyses of Early ZIKV Genomes are Consistent with Viral Spread from Northeast Brazil to The Americas

Laise Eduarda Paixão De Moraes
,
Moyra Machado Portilho
,
Bram Vrancken
,
Frederik Van den Broeck
,
Luciane Amorim Santos
,
Marina Silveira Cucco
,
Laura Beatriz Tauro
,
Mariana Kikuti
,
Monaise Madalena Oliveira Silva
,
Gubio Soares Campos
,
Mitermayer Galvão Reis
,
Aldina Maria Prado Barral
,
Manoel Barral-Netto
ORCID logo ,
Viviane Sampaio Boaventura
,
Anne-Mieke Vandamme
ORCID logo ,
Kristof Theys
ORCID logo ,
Philippe Lemey
,
Guilherme de Sousa Ribeiro
,
Ricardo Khouri
*
Version 1 : Received: 14 April 2023 / Approved: 14 April 2023 / Online: 14 April 2023 (03:51:13 CEST)

A peer-reviewed article of this Preprint also exists.

de Moraes, L.; Portilho, M.M.; Vrancken, B.; Van den Broeck, F.; Santos, L.A.; Cucco, M.; Tauro, L.B.; Kikuti, M.; Silva, M.M.O.; Campos, G.S.; Reis, M.G.; Barral, A.; Barral-Netto, M.; Boaventura, V.S.; Vandamme, A.-M.; Theys, K.; Lemey, P.; Ribeiro, G.S.; Khouri, R. Analyses of Early ZIKV Genomes Are Consistent with Viral Spread from Northeast Brazil to the Americas. Viruses 2023, 15, 1236. de Moraes, L.; Portilho, M.M.; Vrancken, B.; Van den Broeck, F.; Santos, L.A.; Cucco, M.; Tauro, L.B.; Kikuti, M.; Silva, M.M.O.; Campos, G.S.; Reis, M.G.; Barral, A.; Barral-Netto, M.; Boaventura, V.S.; Vandamme, A.-M.; Theys, K.; Lemey, P.; Ribeiro, G.S.; Khouri, R. Analyses of Early ZIKV Genomes Are Consistent with Viral Spread from Northeast Brazil to the Americas. Viruses 2023, 15, 1236.

Abstract

The Americas, particularly Brazil, were greatly impacted by the widespread outbreak of Zika virus (ZIKV) in 2015 and 2016. Efforts were made to implement genomic surveillance of ZIKV as part of the public health responses. The accuracy of spatiotemporal reconstructions of the epidemic spread relies on the unbiased sampling of the transmission process. In the early stages of the outbreak, we recruited patients exhibiting clinical symptoms of arbovirus-like infection from Salvador and Campo Formoso, Bahia, in Northeast Brazil. Between May 2015 and June 2016, we identified 21 cases of acute ZIKV infection and subsequently recovered 14 near full-length sequences using the amplicon tiling multiplex approach with nanopore sequencing. We perform a time-calibrated discrete phylogeographic analysis to trace the spread and migration history of the ZIKV. Our phylogenetic analysis supports a consistent relationship between ZIKV migration from Northeast to Southeast Brazil and its subsequent dissemination beyond Brazil. Additionally, our analysis provides insights into the migration of ZIKV from Brazil to Haiti and the role Brazil played in the spread of ZIKV to other countries, such as Singapore, the USA and Dominican Republic. The data generated by this study enhances our understanding of ZIKV dynamics and supports the existing knowledge, which can aid in future surveillance efforts against the virus.

Keywords

Zika; arboviruses; vector-borne infections; genomic surveillance; phylogenetics

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.