Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Canine Intestinal Organoids as a Novel In Vitro Model of Intestinal Drug Permeability: A Proof-of-Concept Study

Dipak Kumar Sahoo
ORCID logo ,
Marilyn Martinez
* ORCID logo ,
Kimberly Dao
,
Vojtech Gabriel
ORCID logo ,
Christopher Zdyrski
,
Albert E. Jergens
,
Todd Atherly
,
Chelsea A. Iennarella-Servantez
,
Laura E. Burns
,
Dwayne Schrunk
,
Donna A Volpe
,
Karin Allenspach
* ,
Jonathan P. Mochel
* ORCID logo
Version 1 : Received: 31 March 2023 / Approved: 6 April 2023 / Online: 6 April 2023 (14:13:26 CEST)

A peer-reviewed article of this Preprint also exists.

Sahoo, D.K.; Martinez, M.N.; Dao, K.; Gabriel, V.; Zdyrski, C.; Jergens, A.E.; Atherly, T.; Iennarella-Servantez, C.A.; Burns, L.E.; Schrunk, D.; Volpe, D.A.; Allenspach, K.; Mochel, J.P. Canine Intestinal Organoids as a Novel In Vitro Model of Intestinal Drug Permeability: A Proof-of-Concept Study. Cells 2023, 12, 1269. Sahoo, D.K.; Martinez, M.N.; Dao, K.; Gabriel, V.; Zdyrski, C.; Jergens, A.E.; Atherly, T.; Iennarella-Servantez, C.A.; Burns, L.E.; Schrunk, D.; Volpe, D.A.; Allenspach, K.; Mochel, J.P. Canine Intestinal Organoids as a Novel In Vitro Model of Intestinal Drug Permeability: A Proof-of-Concept Study. Cells 2023, 12, 1269.

Abstract

A key component of efforts to identify the biological and drug-specific aspects that contribute to therapeutic failure or unexpected exposure-associated toxicity is the study of drug-intestinal barrier interactions. While methods supporting such assessments are widely described for human therapeutics, there is relatively little information available for similar evaluation in support of veterinary pharmaceuticals. There is, therefore, a critical need to develop novel approaches for evaluating drug-gut interactions in veterinary medicine. Three-dimensional (3D) organoids can address these difficulties in a reasonably affordable system that circumvents the need for more invasive in vivo assays in live animals. However, a first step in the development of such systems is understanding organoid interactions in a 2D monolayer. Given the importance of orally administered medications for meeting the therapeutic need of companion animals, we demonstrate growth conditions under which canine colonoid-derived intestinal epithelial cells survive, mature, and differentiate into confluent cell systems with high monolayer integrity. We further examine the applicability of this canine colonoid-derived 2D model to assess the permeability of three structurally diverse, passively absorbed β-blockers (e.g., propranolol, metoprolol, and atenolol). Both absorptive and secretive apparent permeability (Papp) of these drugs at two different pH conditions were evaluated in canine colonoid-derived monolayers and were compared with that of Caco-2 cells. This proof-of-concept study provides promising preliminary results with regard to the utility of canine-derived organoid monolayers for species-specific assessments of therapeutic drug passive permeability.

Keywords

canine; 3D organoid; permeability; Caco-2; colon

Subject

Medicine and Pharmacology, Veterinary Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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