Embarez, D.H.; Abdel-Razek, A.S.; Basalious, E.B.; Mahmoud, M.; Hamdy, N.M. Acetaminophen-trace Pharmaceutical Bioremediation using Two Isolated Novel Environment-Friendly Phenotypically/Genotypically-characterized Streptomyces Strains; Chemo-informatics & Experimentally tested for Cytotoxicity & Biological Activity. Preprints2023, 2023030121. https://doi.org/10.20944/preprints202303.0121.v1
APA Style
Embarez, D.H., Abdel-Razek, A.S., Basalious, E.B., Mahmoud, M., & Hamdy, N.M. (2023). Acetaminophen-trace Pharmaceutical Bioremediation using Two Isolated Novel Environment-Friendly Phenotypically/Genotypically-characterized Streptomyces Strains; Chemo-informatics & Experimentally tested for Cytotoxicity & Biological Activity. Preprints. https://doi.org/10.20944/preprints202303.0121.v1
Chicago/Turabian Style
Embarez, D.H., Magdy Mahmoud and Nadia M Hamdy. 2023 "Acetaminophen-trace Pharmaceutical Bioremediation using Two Isolated Novel Environment-Friendly Phenotypically/Genotypically-characterized Streptomyces Strains; Chemo-informatics & Experimentally tested for Cytotoxicity & Biological Activity" Preprints. https://doi.org/10.20944/preprints202303.0121.v1
Abstract
We report the isolation of two novel degrading bacterial strains active against the potential environmental pollutant acetamino-phen/paracetamol. Streptomyces Chrestomyceticus (RS2) and Streptomyces Flavofuscus (M33) collected from El-Natrun Valley, Egypt, water, sediment, and sand samples were taxonomically characterized via conidiophores morphologic characterization under Trans-mission Electron Microscope (TEM). Genotypic identification, done based on their 16S rRNA gene sequence analysis followed by BLAST alignment and deposited on the NCBI as 2 novel strains. The phylogenetic tree was constructed using an appropriate software. Acetaminophen degradation-products chemical structure was identified by GC/LC MS. The biological antimicrobial activity of some selected acetaminophen degradation-products extracts and derived compounds was examined against panel of test micro-organisms and found to be have high anti-microbial effect. In addition, in silico cheminformatics Swiss ADMET evaluation of these selected deg-radation extract for gastric absorption, distribution, hepatic metabolism, renal excretion as well as distribution to CNS and finally not mutagenic/teratogenic or genotoxic virtually. In vitro cytotoxic activity of these selected bio-degradation products was performed against HepG2 and MCF7 cancer cell lines, revealing that M33 and RS2 extract effect on acetaminophen/paracetamol bio-degradation products are potentially safe with higher IC50 on HepG2 and MCF7, in comparison to acetaminophen/paracetamol with IC50 of 108.5 μg/ml. Moreover, the in vivo acute oral single dose toxicity experiment was conducted, being compared with acetamino-phen/paracetamol to confirm that the bio-degradation products have lower toxicity than acetaminophen in vivo and in silico.
Biology and Life Sciences, Biochemistry and Molecular Biology
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