Version 1
: Received: 19 February 2023 / Approved: 21 February 2023 / Online: 21 February 2023 (04:21:07 CET)
How to cite:
Scarpati, G.; Corona, M.; Piazza, O. Bile Duct Double Ligation and Microdialysis in Rats: An Animal Model of Liver Fibrosis. Preprints2023, 2023020351. https://doi.org/10.20944/preprints202302.0351.v1
Scarpati, G.; Corona, M.; Piazza, O. Bile Duct Double Ligation and Microdialysis in Rats: An Animal Model of Liver Fibrosis. Preprints 2023, 2023020351. https://doi.org/10.20944/preprints202302.0351.v1
Scarpati, G.; Corona, M.; Piazza, O. Bile Duct Double Ligation and Microdialysis in Rats: An Animal Model of Liver Fibrosis. Preprints2023, 2023020351. https://doi.org/10.20944/preprints202302.0351.v1
APA Style
Scarpati, G., Corona, M., & Piazza, O. (2023). Bile Duct Double Ligation and Microdialysis in Rats: An Animal Model of Liver Fibrosis. Preprints. https://doi.org/10.20944/preprints202302.0351.v1
Chicago/Turabian Style
Scarpati, G., Marco Corona and Ornella Piazza. 2023 "Bile Duct Double Ligation and Microdialysis in Rats: An Animal Model of Liver Fibrosis" Preprints. https://doi.org/10.20944/preprints202302.0351.v1
Abstract
Liver fibrosis is based on complex interactions between extracellular matrix-producing hepatic stellate cells and is defined as excessive matrix deposition and an abundance of infiltrating cells in the liver. Studying these processes requires in vitro and in vivo experimental work on animals. In fibrosis research, experimental work in rodents is currently the gold standard for confirming a proposed disease-associated mechanism. Bile duct ligation (BDL) induces obstructive jaundice resulting in cholestasis. BDL is useful for preclinical research studies of liver injury due to extrahepatic cholestasis such as apoptosis and fibrosis. Here we provide a stepwise surgical approach to resect the common bile duct and monitor in situ hepatic metabolism by microdialysis.
Keywords
liver fibrosis; liver cirrhosis; bile duct ligation; animal model
Subject
Medicine and Pharmacology, Dermatology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
