HAX1 Is a Novel Binding Partner of Che-1/AATF. Implications in Oxidative Stress Cell Response

Cinzia Pisani; Annalisa Onori; Francesca Gabanella; Simona Iezzi; Roberta De Angelis; Maurizio Fanciulli; Andrea Colizza; Marco De Vincentiis; Maria Grazia Di Certo; Claudio Passananti; Nicoletta Corbi

doi:10.20944/preprints202302.0287.v1

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preprints.org > biology and life sciences > biochemistry and molecular biology > doi: 10.20944/preprints202302.0287.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

HAX1 Is a Novel Binding Partner of Che-1/AATF. Implications in Oxidative Stress Cell Response

^* ,

Maria Grazia Di Certo

Claudio Passananti

^* ,

Nicoletta Corbi

Version 1 : Received: 15 February 2023 / Approved: 17 February 2023 / Online: 17 February 2023 (01:50:45 CET)

A peer-reviewed article of this Preprint also exists.

Pisani, C.; Onori, A.; Gabanella, F.; Iezzi, S.; De Angelis, R.; Fanciulli, M.; Colizza, A.; de Vincentiis, M.; Di Certo, M.G.; Passananti, C.; et al. HAX1 Is a Novel Binding Partner of Che-1/AATF. Implications in Oxidative Stress Cell Response. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2024, 1871, 119587, doi:10.1016/j.bbamcr.2023.119587. Pisani, C.; Onori, A.; Gabanella, F.; Iezzi, S.; De Angelis, R.; Fanciulli, M.; Colizza, A.; de Vincentiis, M.; Di Certo, M.G.; Passananti, C.; et al. HAX1 Is a Novel Binding Partner of Che-1/AATF. Implications in Oxidative Stress Cell Response. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2024, 1871, 119587, doi:10.1016/j.bbamcr.2023.119587.

Abstract

HAX1 is a multifunctional protein involved in the antagonism of apoptosis in cellular response to oxidative stress. In the present study we identified HAX1 as novel binding partner for Che-1/AATF, a pro-survival factor which plays a crucial role in fundamental processes, including response to multiple stresses and apoptosis. HAX1 and Che-1 proteins show extensive colocalization in mitochondria and we demonstrated that their association is strengthened after oxidative stress stimuli. Interestingly, in MCF-7 cells, resembling luminal estrogen receptor (ER) positive breast cancer, we found that Che-1 depletion correlates with decreased HAX1 mRNA and protein levels, and this event is not significantly affected by oxidative stress induction. Furthermore, we observed an enhancement of the previously reported interaction between HAX1 and estrogen receptor alpha (ERα) upon H2O2 treatment. These results indicate the two anti-apoptotic proteins HAX1 and Che-1 as coordinated players in cellular response to oxidative stress with a potential role in estrogen sensitive breast cancer cells.

Keywords

HAX1; Che-1/AATF; apoptosis; mitochondria; oxidative stress; breast cancer; ERα

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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