Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

Monique Melo Costa
† ,
Amandine Guidez
† ,
Sébastien Briolant
ORCID logo ,
Stanislas Talaga
,
Jean Issaly
,
Halima Naroua
,
Romuald Carinci
,
Pascal Gaborit
,
Anne Lavergne
,
Isabelle Dusfour
,
Jean-Bernard Duchemin
ORCID logo ,
Lionel Almeras
* ORCID logo
†
These authors contributed equally to this work.
Version 1 : Received: 7 February 2023 / Approved: 8 February 2023 / Online: 8 February 2023 (08:43:03 CET)

A peer-reviewed article of this Preprint also exists.

Costa, M.M.; Guidez, A.; Briolant, S.; Talaga, S.; Issaly, J.; Naroua, H.; Carinci, R.; Gaborit, P.; Lavergne, A.; Dusfour, I.; Duchemin, J.-B.; Almeras, L. Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling. Trop. Med. Infect. Dis. 2023, 8, 168. Costa, M.M.; Guidez, A.; Briolant, S.; Talaga, S.; Issaly, J.; Naroua, H.; Carinci, R.; Gaborit, P.; Lavergne, A.; Dusfour, I.; Duchemin, J.-B.; Almeras, L. Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling. Trop. Med. Infect. Dis. 2023, 8, 168.

Abstract

French Guiana mosquito fauna diversity encompasses 242 species, of which nearly half of them belong to Culex (Diptera: Culicidae) genus. Whereas several species from Culex genus are important vectors of arbovirus, scarce studies focus on Culex genus, notably due to the inaccurate morphological identification of field-caught females gender. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for the identification of mosquitoes. Culex females from French Guiana field were dissected based on morphological classification. Abdomens were used for molecular identification using COI (cytochrome oxydase 1) gene. Legs and thorax of 169 specimens belonging to 13 Culex species,( i.e. Cx. declarator, Cx. nigripalpus, Cx. quinquefasciatus, Cx. usquatus, Cx. adamesi, Cx. dunni, Cx. eastor, Cx. idottus, Cx. pedroi, Cx. phlogistus, Cx. portesi, Cx. rabanicolus and Cx. spissipes) were then submitted to MALDI-TOF MS analysis. A high intra-species reproducibility and inter-species specificity of MS spectra for each mosquito body part tested were noticed. A corroboration of the specimen identification was revealed between MALDI-TOF MS, morphological and molecular results. MALDI-TOF MS protein profiling proves to be a suitable tool for identification of neotropical Culex species and will permit to enhance knowledge on this highly diverse Culex mosquitoes genus.

Keywords

Culex species; neotropical mosquito; French Guiana; MALDI-TOF MS; proteomic tool; arbovirus; monitoring

Subject

Biology and Life Sciences, Insect Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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