Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Novel Strategy to Identify Endolysin With Lytic Activity Against Methicillin-Resistant Staphylococcus aureus

Version 1 : Received: 31 January 2023 / Approved: 3 February 2023 / Online: 3 February 2023 (10:35:34 CET)

A peer-reviewed article of this Preprint also exists.

Kim, H.; Seo, J. A Novel Strategy to Identify Endolysins with Lytic Activity against Methicillin-Resistant Staphylococcus aureus. Int. J. Mol. Sci. 2023, 24, 5772. Kim, H.; Seo, J. A Novel Strategy to Identify Endolysins with Lytic Activity against Methicillin-Resistant Staphylococcus aureus. Int. J. Mol. Sci. 2023, 24, 5772.

Abstract

The increasing prevalence of methicillin-resistant S. aureus (MRSA) in the dairy industry has become a fundamental concern. Endolysins are bacteriophage-derived peptidoglycan hydrolases that induce the rapid lysis of host bacteria. We investigated endolysin candidates with lytic activity against MRSA and evaluated the lytic activity of the endolysin candidate against S. aureus and MRSA. To identify endolysins, we used the following bioinformatic strategy: (1) retrieval of genetic information, (2) annotation, (3) selection of MRSA, (4) selection of endolysin candidates, and (5) evaluation of protein solubility. We then characterized the endolysin candidate under various conditions. Approximately 67% of S. aureus was detected as MRSA and a total of 114 putative endolysins were found. The 114 putative endolysins were divided into three groups based on their combination of conserved domains. Considering the protein solubility, we selected putative endolysins 177 and 117. Putative endolysin 117 was successfully overexpressed and renamed LyJH1892. LyJH1892 lysed approximately 85% of S. aureus. LyJH1892 showed more potent lytic activity against MRSA than normal S. aureus (relative lytic activity > 125%). LyJH1892 showed broad lytic activity against coagulase-negative Staphylococci (CNS). In conclusion, These findings provide a rapid and useful strategy for the development of specific endolysins against antibiotic-resistant bacterial strains.

Keywords

Prophage; Endolysin; Staphylococcus aureus; Methicillin-resistant Staphylococcus aureus; Antibiotic-resistance; Bovine mastitis

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

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