Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by The African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype Against Experimental Challenge with The Pandemic ASFV. Study 2.

Joan Pujols
,
Elena Blazquez
,
Joaquim Segalés
ORCID logo ,
Fernando Rodríguez
ORCID logo ,
Chia-Yu Chang
,
Jordi Argilaguet
,
Laia Bosch-Camós
ORCID logo ,
Rosa Rosell
,
Lola Pailler
,
Boris Gavrilov
,
Joy Campbell
ORCID logo ,
Javier Polo
* ORCID logo
Version 1 : Received: 25 January 2023 / Approved: 31 January 2023 / Online: 31 January 2023 (02:24:23 CET)

A peer-reviewed article of this Preprint also exists.

Pujols, J.; Blázquez, E.; Segalés, J.; Rodríguez, F.; Chang, C.-Y.; Argilaguet, J.; Bosch-Camós, L.; Rosell, R.; Pailler-García, L.; Gavrilov, B.; Campbell, J.; Polo, J. Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2. Vaccines 2023, 11, 825. Pujols, J.; Blázquez, E.; Segalés, J.; Rodríguez, F.; Chang, C.-Y.; Argilaguet, J.; Bosch-Camós, L.; Rosell, R.; Pailler-García, L.; Gavrilov, B.; Campbell, J.; Polo, J. Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2. Vaccines 2023, 11, 825.

Abstract

This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs ac-climated to diets without or with 8% SDPP were intranasally inoculated with 105 plaque forming units (PFU) of live attenuated ASFV strain BA71∆CD2 and three weeks later left in direct contact with pigs infected with the pandemic Georgia2007/01 ASFV strain. During the post-exposure (pe) period, 2/6 from the conventional diet group showed a transient peak rectal temperature >40.5ºC before day 20 pe and some tissue samples collected at 20 d pe from 5/6 were PCR+ for ASFV, albeit showing Ct values much higher than Trojan pigs. Interestingly, the SDPP group did not show fever, neither PCR+ in blood nor rectal swab at any time pe and none of the postmortem collected tissue samples were PCR+ for ASFV. Differential serum cytokine profiles among groups at vaccination, and a higher number of ASFV-specific IFNϒ-secreting T-cells in pigs fed with SDPP soon after the Georgia2007/01 encounter, confirmed the relevance of Th1-like responses in ASF pro-tection. We believe that our result show that nutritional interventions might contribute to improve future ASF vaccination strategies.

Keywords

African swine fever; ASFV; spray-dried porcine plasma; vaccine; challenge; nutritional interven-tion

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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