Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding

Version 1 : Received: 26 January 2023 / Approved: 28 January 2023 / Online: 28 January 2023 (10:30:44 CET)

How to cite: Kalinina, N.; Kostylev, P. Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding. Preprints 2023, 2023010526. https://doi.org/10.20944/preprints202301.0526.v1 Kalinina, N.; Kostylev, P. Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding. Preprints 2023, 2023010526. https://doi.org/10.20944/preprints202301.0526.v1

Abstract

The evaluation of the possibility of obtaining dihaploids by the method of anther culture in vitro to accelerate selection for resistance to prolonged flooding of seeded rice with water was carried out. The object of research is rice hybrids F2 of the Rice Breeding and Seed Production Laboratory of the “Donskoy” ASC, obtained as a result of interbreeding the best economically valuable varieties with samples bearing genes of resistance to prolonged flooding with water. Basic nutrient medium with an optimal composition of nutrition elements and growth hormones stimulating callo- and morphogenesis were used. For the induction of callus formation, 12604 anthers were planted, according to 26 hybrid combinations represented by 68 plants, as a result, 716 neoplasms were obtained, including 586 non–morphogenic callus, 130 regenerating plants. Cultivation of anthers revealed large genotypic differences in the samples. According to the responsiveness to neoplasms, 60% of the panicles showed a positive result, the rest did not give callus. The most responsive to the formation of callus were hybrid combinations: 5009/2 – 84 pcs., 5010/2 – 94 pcs., 4565/3 – 85 pcs., 4641/2 – 69 pcs. They also showed the ability to morphogenesis. Androgenic plants were obtained by 14 hybrid combinations, their share was 1.03% of the total number of inoculated anthers. 30 green regenerative lines from four rice hybrids were obtained, differing in visual morphological assessment: 5009/2 - 5 pcs., 5010/2 – 5 pcs., 4565/3 – 2 pcs., 4641/2 – 18 pcs. The isolated lines are characterized by good responsiveness in the culture of anthers in vitro, carry genes for resistance to prolonged flooding, and can be used in rice breeding programs using DG technologies.

Keywords

rice; variety; hybrid; lines; anther; induction; callus; regeneration; digaploids

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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