Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation, and Enumeration of Bifidobacteria from Breast-Fed, Formula-Fed Infants and Antibiogram Development.

Version 1 : Received: 23 January 2023 / Approved: 23 January 2023 / Online: 23 January 2023 (07:03:40 CET)

How to cite: Kammara, R.; Nellikka, A. Isolation, and Enumeration of Bifidobacteria from Breast-Fed, Formula-Fed Infants and Antibiogram Development.. Preprints 2023, 2023010399. https://doi.org/10.20944/preprints202301.0399.v1 Kammara, R.; Nellikka, A. Isolation, and Enumeration of Bifidobacteria from Breast-Fed, Formula-Fed Infants and Antibiogram Development.. Preprints 2023, 2023010399. https://doi.org/10.20944/preprints202301.0399.v1

Abstract

The population of Bifidobacterium spp in faecal samples from breast-fed and formula-fed infants were investigated and developed antibiogram. The common bacterial growth media such as Man Rogos Sharpe (MRS), Brain Heart Infusion (BHI), Luria Bertani (LB) broth and Bifidobacteria agar were evaluated by the enumeration of Bifidobacterium spp. in faecal material. The results demonstrated that the population of Bifidobacterium spp. in the faecal samples of breast-fed infants is numerically high and, low in formula-fed infants. Phylogenetic analysis of the 16S rRNA, (Xylose/Fructose-6-phosphate phosphoketolase) xfp, and RFLP map of Bifidobacteria isolates suggested a presence of possible new and different Bifidobacterium spp. The intensity of the reddish brown colour formed during the f6ppk (Fructose-6-phosphate phosphoketolase) assay confirms the presence of variable Bifidobacteria. The highest recovery and diversity of Bifidobacteria were obtained from Bifidobacteria agar media. Growth in various media resulted in the formation of small (SCV) and big colony variants (BCV). We understand that the various antibiotic minimum inhibitory concentration (MIC) values obtained by applying different media, growth conditions, after bile salt treatment, and at low pH can vary. This study concludes that the variation of microbiological conditions differentiating resistant and susceptible strains is influenced by the test conditions as well.

Keywords

B. adolescentis; Antibiogram; f6ppk; xfp; RFLP

Subject

Biology and Life Sciences, Food Science and Technology

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