Kim, T.-H.; Yu, G.-R.; Kim, H.; Kim, J.-E.; Lim, D.-W.; Park, W.-H. Efficacy Evaluation of Herbal Prescriptions Related to Hepatic Steatosis by Hyperlipidemia Discovered through Network Pharmacology on FFA-Induced HepG2 Cells. Curr. Issues Mol. Biol.2023, 45, 1314-1332.
Kim, T.-H.; Yu, G.-R.; Kim, H.; Kim, J.-E.; Lim, D.-W.; Park, W.-H. Efficacy Evaluation of Herbal Prescriptions Related to Hepatic Steatosis by Hyperlipidemia Discovered through Network Pharmacology on FFA-Induced HepG2 Cells. Curr. Issues Mol. Biol. 2023, 45, 1314-1332.
Kim, T.-H.; Yu, G.-R.; Kim, H.; Kim, J.-E.; Lim, D.-W.; Park, W.-H. Efficacy Evaluation of Herbal Prescriptions Related to Hepatic Steatosis by Hyperlipidemia Discovered through Network Pharmacology on FFA-Induced HepG2 Cells. Curr. Issues Mol. Biol.2023, 45, 1314-1332.
Kim, T.-H.; Yu, G.-R.; Kim, H.; Kim, J.-E.; Lim, D.-W.; Park, W.-H. Efficacy Evaluation of Herbal Prescriptions Related to Hepatic Steatosis by Hyperlipidemia Discovered through Network Pharmacology on FFA-Induced HepG2 Cells. Curr. Issues Mol. Biol. 2023, 45, 1314-1332.
Abstract
The network pharmacology (NP) approach is a valuable novel methodology for understanding the complex pharmacological mechanisms of medicinal herbs. In addition, various in silico analysis techniques combined with the NP can improve the understanding of various issues in natural product research. This study assessed the therapeutic effects of Arum ternata (AT), Poria cocos (PC), and Zingiber officinale (ZO) on hyperlipidemia after network pharmacologic analysis. A protein–protein interaction (PPI) network of forty-one key targets was analyzed to discover core functional clusters of the herbal compounds. The KEGG pathway and gene ontology (GO) term enrichment analysis identified significant categories of hypolipidemic mechanisms. The STITCH database indicated a high connection with several statin drugs deduced by the similarity in targets. AT, PC, and ZO regulated the genes related to the energy metabolism and lipogenesis in HepG2 cells loaded with free fatty acids (FFAs). Furthermore, a combinational effect of the mixture of three herbs was found. The herbal combination exerted superior efficacy compared to a single herb, particularly in regulating acetyl-CoA carboxylase (ACC) and carnitine palmitoyltransferase 1 (CPT-1). In conclusion, the network pharmacologic approach was used to assess potential targets of the herbal combination for treatment. Experimental data from FFAs-induced HepG2 cells suggested that the combination of AT, PC, and ZO might attenuate hyperlipidemia and its associated hepatic steatosis.
Medicine and Pharmacology, Pharmacology and Toxicology
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