Ji, Y.; Zhang, H.; Wang, Y.; Liu, B.; Lu, T.; Wang, Z. Grass Endophyte Researches 21: The Fungal Endophyte within Stroma-Developed Leymus Chinensis: Mycological Properties and Taxonomic Discrimination. Preprints2023, 2023010114. https://doi.org/10.20944/preprints202301.0114.v1
APA Style
Ji, Y., Zhang, H., Wang, Y., Liu, B., Lu, T., & Wang, Z. (2023). Grass Endophyte Researches 21: The Fungal Endophyte within Stroma-Developed <i>Leymus Chinensis</i>: Mycological Properties and Taxonomic Discrimination. Preprints. https://doi.org/10.20944/preprints202301.0114.v1
Chicago/Turabian Style
Ji, Y., Tao Lu and Zhiwei Wang. 2023 "Grass Endophyte Researches 21: The Fungal Endophyte within Stroma-Developed <i>Leymus Chinensis</i>: Mycological Properties and Taxonomic Discrimination" Preprints. https://doi.org/10.20944/preprints202301.0114.v1
Abstract
During Sep. 2011, forty-four Leymus chinensis culms with stroma development were collected from Inner Mongolia. Stromata surrounded the flag leaf sheathes, white, at the final stage of development. Perithecium but not asci were observed under microscope. Nine epichloid endophyte strains were obtained from Tongliao subdivision, and sixteen from Horqin. Morphological and phylogenetic properties of these strains were investigated. Morphological properties of these strains were similar among these 27 strains. Colonies could grow to 28.5~41.1 mm/21d at 25℃; phialides 24.1~26.4 μm long, 2.1~2.5 μm at base, tapering to less than 1.0 μm at tip; conidia reniform to elliptical, 4.7~5.5×2.8~3.5 μm, indicating typical properties of Epichloë endophytes. Based on the sequences of tefA and tubB gene fragments, strains isolated from Inner Mongolia formed a distinct subclade in E. bromolica/ E. yangzii clade, with a bootstrap value of 66% and 64% respectively. Considering morphological and phylogenetic properties, we tend to determine strains isolated from Leymus chinensis as a new taxon. The detailed taxonomic position of this taxon needs further investigations on hybridization or genomic sequence.
Biology and Life Sciences, Immunology and Microbiology
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