Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Ubiquitin-binding Autophagic Receptors in Yeast: Cue5 and beyond

Version 1 : Received: 30 December 2022 / Approved: 5 January 2023 / Online: 5 January 2023 (04:33:12 CET)

How to cite: Mensah, T.N.A.; Shroff, A.; Nazarko, T.Y. Ubiquitin-binding Autophagic Receptors in Yeast: Cue5 and beyond. Preprints 2023, 2023010099 (doi: 10.20944/preprints202301.0099.v1). Mensah, T.N.A.; Shroff, A.; Nazarko, T.Y. Ubiquitin-binding Autophagic Receptors in Yeast: Cue5 and beyond. Preprints 2023, 2023010099 (doi: 10.20944/preprints202301.0099.v1).

Abstract

The selectivity in selective autophagy pathways is achieved via the selective autophagy receptors (SARs) – proteins that bind a ligand on the substrate to be degraded and the Atg8-family protein on the growing autophagic membrane, phagophore, effectively bridging them. In mammals, the most common ligand of SARs is ubiquitin, a small protein modifier that tags substrates for their preferential degradation by autophagy. Consequently, the most common SARs are the ubiquitin-binding SARs, such as SQSTM1/p62 (sequestosome 1). Surprisingly, there is only one SAR of this type in yeast – Cue5, which acts as a receptor for aggrephagy and proteaphagy – pathways that remove the ubiquitinated protein aggregates and proteasomes, respectively. However, recent studies described the ubiquitin-dependent autophagic pathways that do not require Cue5, e.g. stationary phase lipophagy for intracellular lipid droplets and nitrogen starvation-induced mitophagy for mitochondria. What is the role of ubiquitin in these pathways? Here, we propose that the ubiquitinated lipid droplets and mitochondria are recognized by the alternative ubiquitin-binding SARs. Our analysis identifies the proteins that could potentially fulfill this role in yeast. We think that matching of the ubiquitin-dependent (but Cue5-independent) autophagic pathways with the ubiquitin-and-Atg8-binding proteins enlisted here might uncover the novel ubiquitin-binding SARs in yeast.

Keywords

Atg8; autophagic receptor; Cue5; SAR; selective autophagy; selective autophagy receptor; ubiquitin; ubiquitin-binding domain; ubiquitin-binding protein; ubiquitin-binding receptor

Subject

LIFE SCIENCES, Cell & Developmental Biology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.