Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Insights into the Phytopathogenicity of Alternaria alternata Isolated from Infected Pears (Pyrus communis) in Italy: Enzymatic Activity and Production of Non-Host Toxins

Stefany Castaldi
ORCID logo ,
Jesús Garcia Zorrilla
ORCID logo ,
Claudia Petrillo
,
Maria Teresa Russo
,
Patrizia Ambrosino
,
Marco Masi
ORCID logo ,
Alessio Cimmino
ORCID logo ,
Rachele Isticato
* ORCID logo
Version 1 : Received: 22 December 2022 / Approved: 30 December 2022 / Online: 30 December 2022 (01:57:03 CET)

A peer-reviewed article of this Preprint also exists.

Castaldi, S.; Zorrilla, J.G.; Petrillo, C.; Russo, M.T.; Ambrosino, P.; Masi, M.; Cimmino, A.; Isticato, R. Alternaria alternata Isolated from Infected Pears (Pyrus communis) in Italy Produces Non-Host Toxins and Hydrolytic Enzymes as Infection Mechanisms and Exhibits Competitive Exclusion against Botrytis cinerea in Co-infected Host Fruits. J. Fungi 2023, 9, 326. Castaldi, S.; Zorrilla, J.G.; Petrillo, C.; Russo, M.T.; Ambrosino, P.; Masi, M.; Cimmino, A.; Isticato, R. Alternaria alternata Isolated from Infected Pears (Pyrus communis) in Italy Produces Non-Host Toxins and Hydrolytic Enzymes as Infection Mechanisms and Exhibits Competitive Exclusion against Botrytis cinerea in Co-infected Host Fruits. J. Fungi 2023, 9, 326.

Abstract

This lack of information is due to the primary role of HSTs in fungal pathogenesis, which often masks the functions of NSTs and CWDEs. So, the toxic effects of A. alternata metabolites due to NSTs and CWDEs have received minor attention than those reported for HSTs mycotoxins [11]. A wider study of the activity of isolated fungal metabolites can allow the identification of compounds directly related to the pathogenic activity of the fungus, making it possible to create chemo libraries that facilitate the linking of the structure of the compounds with the species that produce it and its effect on host and non-host crops, as well as with biosynthetic features [12]. In this context, our work reports a study focused on NSTs and CWDEs used by an A. alternata strain isolated from infected pears in Italy. To this aim, the characterization of hydrolytic enzyme activities of A. alternata and the identification of the metabolites produced in vitro were performed. Furthermore, the phytotoxic activity of the isolated compounds was evaluated on pear (host and non-host varieties) and lemon fruits [13]. Finally, the competition of A. alternata with other pathogens was evaluated to investigate the role of NSTs on co-infections.

Keywords

Alternaria alternata; pear pathogens; multi-infections; enzymatic activities; mycotoxins; plant necrosis.

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.