Version 1
: Received: 15 December 2022 / Approved: 21 December 2022 / Online: 21 December 2022 (04:32:57 CET)
How to cite:
Sir El-Khatim Ali, K.; Daffalla, H. M.; Osman, M. G.; Musa, A. Germination and Early Growth of Acacia nubica Benth. In vitro. Preprints2022, 2022120385. https://doi.org/10.20944/preprints202212.0385.v1
Sir El-Khatim Ali, K.; Daffalla, H. M.; Osman, M. G.; Musa, A. Germination and Early Growth of Acacia nubica Benth. In vitro. Preprints 2022, 2022120385. https://doi.org/10.20944/preprints202212.0385.v1
Sir El-Khatim Ali, K.; Daffalla, H. M.; Osman, M. G.; Musa, A. Germination and Early Growth of Acacia nubica Benth. In vitro. Preprints2022, 2022120385. https://doi.org/10.20944/preprints202212.0385.v1
APA Style
Sir El-Khatim Ali, K., Daffalla, H. M., Osman, M. G., & Musa, A. (2022). Germination and Early Growth of <em>Acacia nubica </em>Benth.<em> In vitro</em>. Preprints. https://doi.org/10.20944/preprints202212.0385.v1
Chicago/Turabian Style
Sir El-Khatim Ali, K., Magdoleen G. Osman and Abdulahafiz Musa. 2022 "Germination and Early Growth of <em>Acacia nubica </em>Benth.<em> In vitro</em>" Preprints. https://doi.org/10.20944/preprints202212.0385.v1
Abstract
Acacia nubica is well-adapted to harsh conditions and offers valued socio-economic and environmental benefits. This research studied seed germination and seedling growth of A. nubicain vitro in response to the application of different concentrations of sodium hypochlorite, sucrose, and Murashige and Skoog (MS) medium. The synchrony, percentage, and rate of germination of A. nubica seed were studied. Seedling development was assessed by measuring the shoot, root, and number of nodes. Sodium hypochlorite at 10% produced the maximum germination percentage, while 5% induced a faster and more uniform emergence. Sucrose has various effects onseedemergence and germination depending on the concentrations; 30 g/L elicited thehighest germination, while 10 g/L improved seed emergence rate with higher uniformity. The highest seed germination was achieved on ¼-MS medium, although 0-MS strength recorded faster and more synchronized germination. The development of A. nubica seedlings was influenced by MS medium strengths and sucrose concentrations. Full MS medium produced the highest shoot length and the highest vigor index with the addition of 20 g/L sucrose and the maximum root length with 50 g/L sucrose. Also, 30 g/L sucrose gave the maximum number of nodes and the maximum root/shoot ratio. However, ½-MS strength yields the highest seedling biomass with 30 g/L sucrose. This is the first successful report on the seed germination and seedling growth of A. nubica in vitro.
Keywords
Acacia nubica; dormancy; germination; in vitro; seedling; synchronization
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
