Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

2-Isobutyl-3-methoxypyrazine as a Putative Male-Specific Aggregation Pheromone in Labidostomis lusitanica (Germar) (Coleoptera: Chrysomelidae)

Sergio López
* ORCID logo ,
Sara Rodrigo-Gómez
,
Enrique Fernández-Carrillo
,
Cláudia Corbella-Martorell
,
Carmen Quero
* ORCID logo
Version 1 : Received: 16 December 2022 / Approved: 20 December 2022 / Online: 20 December 2022 (01:41:47 CET)

A peer-reviewed article of this Preprint also exists.

López, S.; Rodrigo-Gómez, S.; Fernández-Carrillo, E.; Corbella-Martorell, C.; Quero, C. Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in Labidostomis lusitanica (Germar) (Coleoptera: Chrysomelidae). Insects 2023, 14, 107. López, S.; Rodrigo-Gómez, S.; Fernández-Carrillo, E.; Corbella-Martorell, C.; Quero, C. Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in Labidostomis lusitanica (Germar) (Coleoptera: Chrysomelidae). Insects 2023, 14, 107.

Abstract

In spite of its incidence on pistachio trees, the chemical ecology of Labidostomis lusitanica (Germar) (Coleoptera: Chrysomelidae) has been neglected so far. In this work we provide the first evidence of a biologically active male-specific compound that may be promoting field aggregation. Headspace collections by solid-phase microextraction from feral males and females reported the presence of 2-isobutyl-3-methoxypyrazine exclusively on males. Electroantennographic recordings revealed that males and females responded in a dose-dependent manner to increasing stimuli of 2-isobutyl-3-methoxypyrazine, with females overall displaying a higher response than males. In dual-choice tests, both males and females showed a significant preference for the compound in comparison to a pure air stimulus. In the light of these results, the possible role of 2-isobutyl-3-methoxypyrazine as an aggregation cue in L. lusitanica is discussed.

Keywords

Labidostomis lusitanica; Chrysomelidae; Pistacia vera; 2-isobutyl-3-methoxypyrazine; electroantennography; behavior

Subject

Biology and Life Sciences, Insect Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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