Version 1
: Received: 16 November 2022 / Approved: 21 November 2022 / Online: 21 November 2022 (03:36:19 CET)
How to cite:
Bucciarelli, A. Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration. Preprints2022, 2022110366. https://doi.org/10.20944/preprints202211.0366.v1
Bucciarelli, A. Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration. Preprints 2022, 2022110366. https://doi.org/10.20944/preprints202211.0366.v1
Bucciarelli, A. Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration. Preprints2022, 2022110366. https://doi.org/10.20944/preprints202211.0366.v1
APA Style
Bucciarelli, A. (2022). Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration. Preprints. https://doi.org/10.20944/preprints202211.0366.v1
Chicago/Turabian Style
Bucciarelli, A. 2022 "Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration" Preprints. https://doi.org/10.20944/preprints202211.0366.v1
Abstract
Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light. This allows the structuring of this material throught additive manufacturing techniques and then to easily prototype patient specific construct. In this study we used Sil-MA to produce single layer crosslinked structures that can be withdrawal and ejected recovering their shape after rehydration. A complete chemical and physical characterization of the material has been conducted. Additionally, we tested the material biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility to use it in future trials. The material was also tested to verify its ability to support the osteogenesis. Two different additive manufacturing techniques have been tested (a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique) to develop Sil-MA grid. Finally, we provide a proof-of-concept that the printed Sil-MA structures are injectable.
Keywords
Silk Fibroin; Sil-MA; 3D Printing; Additive Manufacturing; Tissue Engineering; Bioprinting; Bone Tissue Engineering
Subject
Chemistry and Materials Science, Materials Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
