Version 1
: Received: 9 November 2022 / Approved: 14 November 2022 / Online: 14 November 2022 (11:08:08 CET)
How to cite:
Strižincová, P.; Šurina, I.; Majová, V.; Ház, A.; Hroboňová, K. Choline Chloride: Lactic Acid as a Green Solvent for the Extraction of Eugenol, Eugenol Acetate, and β-Caryophyllene of Clove Buds. Preprints2022, 2022110256. https://doi.org/10.20944/preprints202211.0256.v1
Strižincová, P.; Šurina, I.; Majová, V.; Ház, A.; Hroboňová, K. Choline Chloride: Lactic Acid as a Green Solvent for the Extraction of Eugenol, Eugenol Acetate, and β-Caryophyllene of Clove Buds. Preprints 2022, 2022110256. https://doi.org/10.20944/preprints202211.0256.v1
Strižincová, P.; Šurina, I.; Majová, V.; Ház, A.; Hroboňová, K. Choline Chloride: Lactic Acid as a Green Solvent for the Extraction of Eugenol, Eugenol Acetate, and β-Caryophyllene of Clove Buds. Preprints2022, 2022110256. https://doi.org/10.20944/preprints202211.0256.v1
APA Style
Strižincová, P., Šurina, I., Majová, V., Ház, A., & Hroboňová, K. (2022). Choline Chloride: Lactic Acid as a Green Solvent for the Extraction of Eugenol, Eugenol Acetate, and β-Caryophyllene of Clove Buds. Preprints. https://doi.org/10.20944/preprints202211.0256.v1
Chicago/Turabian Style
Strižincová, P., Aleš Ház and Katarína Hroboňová. 2022 "Choline Chloride: Lactic Acid as a Green Solvent for the Extraction of Eugenol, Eugenol Acetate, and β-Caryophyllene of Clove Buds" Preprints. https://doi.org/10.20944/preprints202211.0256.v1
Abstract
In this study, the effect of extraction conditions on the total phenolic content of clove buds was investigated. Clove buds were extracted using a deep eutectic solvents like mixtures choline chloride: lactic acid in the 1:2 molar ratio. Additionally, the HPLC-DAD method was used to determine the main components of the clove extracts, including eugenol, eugenol acetate, and β-caryophyllene. The conditions used in the extraction with choline chloride: lactic acid (molar ratio 1:2) included extraction temperature (t = 40 – 80°C), water addition (5.6 – 40%), and extraction time (30 – 90 min). Determination of the phenolic compounds was done by the Folin-Ciocalteu method. The optimum operating conditions for the total phenolic content were identified at 77°C, 30 min, and a water addition of 40%. Based on the results, the highest amount of eugenol (307.26 ± 8,44 mg/g dry raw material) was determined in clove extracts which were extracted under condition of 60°C, 22.8%, and 30 min.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
